Cell mediated immunity induced in mice by HPV 16 L1 virus-like particles

被引：45
作者：
Dupuy, C [1 ]
BuzoniGatel, D [1 ]
Touze, A [1 ]
LeCann, P [1 ]
Bout, D [1 ]
Coursaget, P [1 ]
机构：
[1] FAC PHARM TOURS, LAB IMMUNOL MALAD INFECT, F-37200 TOURS, FRANCE
来源：
MICROBIAL PATHOGENESIS | 1997年 / 22卷 / 04期
关键词：
HPV; cervical cancer; virus-like particles; cell mediated immunity; cytokines; Th1; profile;
D O I：
10.1006/mpat.1996.0113
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Recombinant human papillomavirus (HPV) type 16 L1 virus-like particles (VLPs) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. The cell-mediated immune response was evaluated through immunization of mice with HPV 16 L1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. A significant proliferative response was observed which was associated with secretion of both interferon-gamma and interleukin-2. FAGS analysis of splenic lymphocytes revealed that CD8(+) T-cells were increased in the immunized mice. These results demonstrate that HPV 16 L1 VLPs induce a T-cell response characterized by a Th1 profile and confirm that the HPV 16 VLP is a reasonable candidate for vaccine development. (C) 1997 Academic Press Limited.
引用
收藏
页码：219 / 225
页数：7
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