Cell mediated immunity induced in mice by HPV 16 L1 virus-like particles

被引：45

作者：

Dupuy, C ^{[1
]}

BuzoniGatel, D ^{[1
]}

Touze, A ^{[1
]}

LeCann, P ^{[1
]}

Bout, D ^{[1
]}

Coursaget, P ^{[1
]}

机构：

[1] FAC PHARM TOURS, LAB IMMUNOL MALAD INFECT, F-37200 TOURS, FRANCE

来源：

MICROBIAL PATHOGENESIS | 1997年 / 22卷 / 04期

关键词：

HPV; cervical cancer; virus-like particles; cell mediated immunity; cytokines; Th1; profile;

D O I：

10.1006/mpat.1996.0113

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recombinant human papillomavirus (HPV) type 16 L1 virus-like particles (VLPs) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. The cell-mediated immune response was evaluated through immunization of mice with HPV 16 L1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. A significant proliferative response was observed which was associated with secretion of both interferon-gamma and interleukin-2. FAGS analysis of splenic lymphocytes revealed that CD8(+) T-cells were increased in the immunized mice. These results demonstrate that HPV 16 L1 VLPs induce a T-cell response characterized by a Th1 profile and confirm that the HPV 16 VLP is a reasonable candidate for vaccine development. (C) 1997 Academic Press Limited.

引用

页码：219 / 225

页数：7

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