Characterization of rat CD4 T cell clones specific for the major surface glycoprotein of Pneumocystis carinii

被引:10
作者
Theus, SA
Andrews, RP
Linke, MJ
Walzer, PD
机构
[1] VET AFFAIRS MED CTR,RES SERV,CINCINNATI,OH 45220
[2] UNIV CINCINNATI,DEPT INTERNAL MED,DIV INFECT DIS,CINCINNATI,OH 45267
关键词
antigens; infectious disease; pneumonia;
D O I
10.1111/j.1550-7408.1997.tb05944.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pneumocystis carinii are coated by abundant heterogenous major surface glycoproteins (MSGs), which facilitate interaction with the host. We have produced MSG-specific T-cell clones from the spleens of P, carinii-exposed Lewis rats and analyzed five for antigen specificity to native MSG and a recombinant form of MSG, cell surface markers, and cytokine profiles. All five of the clones were CD4+. All of the clones proliferated specifically to both the native MSG and the recombinant MSG only in the presence of antigen presenting cells demonstrating that the response is antigen/driven rather than mitogen/driven. All five of the clones secreted IL-2 and IFN-gamma, although in differing amounts, implicating a Th1 response. Only one of the clones produced any detectable IL-4. This is the first report of T cell clones responsive to a specific antigen of P. carinii, MSG. We conclude that the T cell clones will be helpful in mapping protective epitopes present in MSG and in functional studies of MSG.
引用
收藏
页码:96 / 100
页数:5
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