Altered Expression of Type-1 and Type-2 Cannabinoid Receptors in Celiac Disease

被引：11

作者：

Battista, Natalia ^{[1
,2
]}

Di Sabatino, Antonio ^{[3
]}

Di Tommaso, Monia ^{[1
]}

Biancheri, Paolo ^{[3
]}

Rapino, Cinzia ^{[1
]}

Giuffrida, Paolo ^{[3
]}

Papadia, Cinzia ^{[4
]}

Montana, Chiara ^{[4
]}

Pasini, Alessandra ^{[3
]}

Vanoli, Alessandro ^{[5
]}

Lanzarotto, Francesco ^{[6
]}

Villanacci, Vincenzo ^{[7
]}

Corazza, Gino R. ^{[3
]}

Maccarrone, Mauro ^{[2
,8
]}

机构：

[1] Univ Teramo, Dept Biomed Sci, Teramo, Italy

[2] Santa Lucia Fdn, European Ctr Brain Res CERC, Rome, Italy

[3] Univ Pavia, Dept Internal Med, Fdn IRCCS Policlin S Matteo, Ctr Studio & Cura Malattia Celiaca, I-27100 Pavia, Italy

[4] Parma Univ Hosp, Gastroenterol Unit, Parma, Italy

[5] Univ Pavia, Dept Mol Med, Fdn IRCCS Policlin S Matteo, Ctr Studio & Cura Malattia Celiaca, I-27100 Pavia, Italy

[6] Spedali Civil Brescia, Dept Gastroenterol, I-25125 Brescia, Italy

[7] Spedali Civil Brescia, Dept Pathol, I-25125 Brescia, Italy

[8] Campus Biomed Univ Rome, Ctr Integrated Res, Rome, Italy

来源：

PLOS ONE | 2013年 / 8卷 / 04期

关键词：

IRRITABLE-BOWEL-SYNDROME; ENDOCANNABINOID SYSTEM; PHARMACOGENETIC TRIAL; GENE POLYMORPHISM; ACTIVATION; GUT; CB1; PHENOTYPE; COLITIS; CELLS;

D O I：

10.1371/journal.pone.0062078

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Anandamide (AEA) is the prominent member of the endocannabinoid family and its biological action is mediated through the binding to both type-1 (CB1) and type-2 (CB2) cannabinoid receptors (CBR). The presence of AEA and CBR in the gastrointestinal tract highlighted their pathophysiological role in several gut diseases, including celiac disease. Here, we aimed to investigate the expression of CBR at transcriptional and translational levels in the duodenal mucosa of untreated celiac patients, celiac patients on a gluten-free diet for at least 12 months and control subjects. Also biopsies from treated celiac patients cultured ex vivo with peptic-tryptic digest of gliadin were investigated. Our data show higher levels of both CB1 and CB2 receptors during active disease and normal CBR levels in treated celiac patients. In conclusion, we demonstrate an up-regulation of CB1 and CB2 mRNA and protein expression, that points to the therapeutic potential of targeting CBR in patients with celiac disease.

引用

页数：6

共 31 条

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Alhouayek M, 2012, TRENDS MOL IN PRESS

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