Binding affinities of the SH2 domains of ZAP-70, p(56)lck and Shc to the zeta chain ITAMs of the T-cell receptor determined by surface plasmon resonance

The zeta chains of the T cell receptor complex play a critical role in the initiation of proximal signaling events upon T cell activation, Three pairs of potential tyrosine phosphorylation sites are located within the cytoplasmic domains of the zeta chains, Subsequent to engagement of the T cell receptor, one or more of these tyrosine residues is phosphorylated, Tbe phosphotyrosine residues, along with flanking amino acids, form an activation motif (and are shared by signaling subunits in the TCR, B cell receptor, and Fc gamma RI) termed tyrosine-based activation motifs (ITAMs). ITAMs serve as binding sites for SH2 domain-containing proteins, Recent evidence suggests that the zeta chains provide docking space for several key signal transduction molecules such as ZAP-70, p(56)lck, and Shc. To determine if ZAP-70, p(56)lck, and She bind to particular zeta chain ITAM sequences, quantitative free-solution measurements of binding affinities (K-d) were obtained by use of surface plasmon resonance technology, The results indicate that binding affinities of distinct SH2 domains to individual and paired phosphorylation sites greatly differ, and may dictate the sequence of signal transduction events.