Injection of antisense oligos to nNOS into nucleus tractus solitarii increases blood pressure

被引：27

作者：

Maeda, M

Hirano, H

Kudo, H

Doi, Y

Higashi, K

Fujimoto, S

机构：

[1] Univ Occupat & Environm Hlth, Sch Med, Inst Ind Ecol Sci, Dept Syst Physiol,Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

[2] Univ Occupat & Environm Hlth, Sch Med, Dept Biochem, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

[3] Univ Occupat & Environm Hlth, Sch Med, Dept Anat, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

来源：

NEUROREPORT | 1999年 / 10卷 / 09期

关键词：

antisense; blood pressure; heart rate; neuronal nitric oxide synthase; nitric oxide; nucleus tractus solitarii; oligonucleotides; oligos; rat;

D O I：

10.1097/00001756-199906230-00030

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WE investigated the cardiovascular effects of bilateral microinjection of antisense oligodeoxynucleotides (oligos) into the nucleus tractus solitarii (NTS) to neuronal nitric oxide synthase (nNOS) to suppress the expression of nNOS molecular biologically. In urethane-anesthetized, paralyzed Wistar-Kyoto rats, bilateral microinjection of nNOS antisense oligos (20 pmol in a 50 nl volume) into the NTS produced a significant increase in mean arterial blood pressure at 30-60 min after injection, compared with rats injected with nNOS sense or scrambled oligos. Immunohistochemical study demonstrated that nNOS immunoreactivity in the rat NTS was suppressed by nNOS antisense oligos. These results indicate that suppression of the nNOS gene using antisense in the NTS increases blood pressure. NeuroReport 10:1957-1960 (C) 1999 Lippincott Williams & Wilkins.

引用

页码：1957 / 1960

页数：4

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