Enhancement of Skin Wound Healing by rhEGF-Loaded Carboxymethyl Chitosan Nanoparticles

被引:22
作者
Zhang, Pei [1 ,2 ]
Liu, Chenguang [1 ]
机构
[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
[2] Luoyang Normal Univ, Dept Life Sci, Luoyang 471934, Peoples R China
基金
中国国家自然科学基金;
关键词
carboxymethyl chitosan; conjugated linoleic acid; self-assembly; nanoparticles; cytotoxicity; rhEGF; migration; re-epithelialization; EPIDERMAL-GROWTH-FACTOR; IN-VITRO; CONTROLLED-RELEASE; STABILITY; EGF; PHENYLALANINE; BIOACTIVITY; ADSORPTION; CONJUGATE; DELIVERY;
D O I
10.3390/polym12071612
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
The self-assembly of hydrophobically modified polymers has become a research hotspot due to its wide application in the biomedical field. Recombinant human epidermal growth factors (rhEGFs) are molecules that are able to enhance wound healing; however, they have a short half-life and require sustained action to enhance their mitogenic effect on epithelial cells. Here, we proposed a new delivery system to avoid the inhibition of rhEGF by various enzymes, thus improving its bioavailability and sustained release. The amphiphilic polymer was composed of conjugated linoleic acid (CLA) and carboxymethyl chitosan (CMCS), which were further characterized by fourier transformed infrared spectroscopy (FTIR) and(1)H nuclear magnetic resonance (H-1 NMR). Then, the self-assembly behavior of CLA-CMCS (CC) polymer in water was observed in which the particle size of CC decreased from 196 to 155 nm with the degree of CLA substitution increasing. The nanoparticles were loaded with rhEGF and the maximum rhEGF loading efficiency (LE) of CC3 nanoparticles was 82.43 +/- 3.14%. Furthermore, CC nanoparticles (NPs) exhibited no cytotoxicity for L929 cells, and cell proliferation activity was well preserved after rhEGF loading to CC-NPs and was comparable to that of free rhEGF. Topically applied rhEGF:CC-NPs significantly accelerated the wound-closure rate in full thickness, which was most probably due to its sustained release and enhanced skin permeation. In conclusion, carboxymethyl chitosan-based nanoparticles were constructed and showed good cytocompatibility. Moreover, these findings also demonstrated the therapeutic potential of rhEGF:CC-NPs as a topical wound-healing drug carrier.
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页数:14
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