Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions

被引：34

作者：

Kim, Yonghwi ^{[1
]}

Chung, Kyungwha ^{[2
]}

Lee, Wonju ^{[1
]}

Kim, Dong Ha ^{[2
]}

Kim, Donghyun ^{[1
]}

机构：

[1] Yonsei Univ, Sch Elect & Elect Engn, Seoul 120749, South Korea

[2] Ewha Womans Univ, Dept Chem & Nano Sci, Div Mol & Life Sci, Coll Nat Sci, Seoul 120750, South Korea

来源：

APPLIED PHYSICS LETTERS | 2012年 / 101卷 / 23期

基金：

新加坡国家研究基金会;

关键词：

CONJUGATED DNA HYBRIDIZATION; BIOSENSORS; IMMOBILIZATION; NANOSTRUCTURES; ENHANCEMENT; SENSORS;

D O I：

10.1063/1.4769108

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We have performed highly sensitive surface plasmon resonance (SPR) detection by colocalizing the evanescent near-fields and target molecular distribution. The colocalization is based on oblique metal evaporation to form nanogaps of a size under 100nm without using electron-beam lithography. The concept was demonstrated by detecting siloxane-based biotin/streptavidin interactions. 50-nm nanogaps produced the largest amplification of optical signatures and two orders of magnitude enhancement of sensitivity over conventional thin film-based measurements. The enhancement is associated with efficient overlap of localized near-fields and target. Colocalized detection scheme is expected to provide clues to molecular sensitivity for SPR biosensing. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4769108]

引用

页数：5

共 30 条

[1] Design study of highly sensitive nanowire-enhanced surface plasmon resonance biosensors using rigorous coupled wave analysis [J].

Byun, KM ;

Kim, SJ ;

Kim, D .

OPTICS EXPRESS, 2005, 13 (10) :3737-3742

[2] Experimental study of sensitivity enhancement in surface plasmon resonance biosensors by use of periodic metallic nanowires [J].

Byun, Kyung Min ;

Yoon, Soon Joon ;

Kim, Donghyun ;

Kim, Sung June .

OPTICS LETTERS, 2007, 32 (13) :1902-1904

[3] Effect of target localization on the sensitivity of a localized surface plasmon resonance biosensor based on subwavelength metallic nanostructures [J].

Byun, Kyung Min ;

Jang, Seong Min ;

Kim, Sung June ;

Kim, Donghyun .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2009, 26 (04) :1027-1034

[4] SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics [J].

Campbell, Charles T. ;

Kim, Gibum .

BIOMATERIALS, 2007, 28 (15) :2380-2392

[5] Enhanced sensitivity of self-assembled-monolayer-based SPR immunosensor for detection of benzaldehyde using a single-step multi-sandwich immunoassay [J].

Gobi, K. Vengatajalabathy ;

Matsumoto, Kiyoshi ;

Toko, Kiyoshi ;

Ikezaki, Hidekazu ;

Miura, Norio .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (08) :2727-2735

[6] Chemical modification of self-assembled silane based monolayers by surface reactions [J].

Haensch, Claudia ;

Hoeppener, Stephanie ;

Schubert, Ulrich S. .

CHEMICAL SOCIETY REVIEWS, 2010, 39 (06) :2323-2334

[7] Surface Plasmon Resonance Phase Imaging Measurements of Patterned Mono layers and DNA Adsorption onto Microarrays [J].

Halpern, Aaron R. ;

Chen, Yulin ;

Corn, Robert M. ;

Kim, Donghyun .

ANALYTICAL CHEMISTRY, 2011, 83 (07) :2801-2806

[8] Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization [J].

He, L ;

Musick, MD ;

Nicewarner, SR ;

Salinas, FG ;

Benkovic, SJ ;

Natan, MJ ;

Keating, CD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9071-9077

[9] Multifunctional Polypeptide EQCN Sensors: Probing the Cysteamine-Glutathione Film Permeability with Hg(II) Ions [J].

Hepel, Maria ;

Dallas, Julia .

SENSORS, 2008, 8 (11) :7224-7240

[10] Enhanced SPR response from patterned immobilization of surface bioreceptors on nano-gratings [J].

Hoa, X. D. ;

Kirk, A. G. ;

Tabrizian, M. .

BIOSENSORS & BIOELECTRONICS, 2009, 24 (10) :3043-3048

← 1 2 3 →