Effect of phospholipid insertion on arrayed polydiacetylene biosensors

被引：52

作者：

Kim, Kyung-Woo

Choi, Hyun

Lee, Gil Sun

Ahn, Dong June ^{[1
]}

Oh, Min-Kyu

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136713, South Korea

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 66卷 / 02期

关键词：

polydiacetylene; dimyristoyl phosphatidylcholine (DMPC); FTIR; label-free biosensor; bacteria sensor;

D O I：

10.1016/j.colsurfb.2008.06.020

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis Suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：213 / 217

页数：5

共 27 条

[1] Colorimetric reversibility of polydiacetylene supramolecules having enhanced hydrogen-bonding under thermal and pH stimuli [J].