Histological and immunohistochemical evaluation of the peri-implant soft tissues around machined and acid-etched titanium healing abutments: a prospective randomised study

被引:45
作者
Degidi, Marco [1 ]
Artese, Luciano [1 ]
Piattelli, Adriano [1 ]
Scarano, Antonio [1 ]
Shibli, Jamil A. [2 ,3 ]
Piccirilli, Marcello [1 ]
Perrotti, Vittoria [1 ]
Iezzi, Giovanna [1 ]
机构
[1] Univ G dAnnunzio, Sch Dent, I-66100 Chieti, Italy
[2] Univ Guarulhos, Dept Periodontol, Dent Res Div, Sao Paulo, Brazil
[3] Univ Guarulhos, Oral Implantol Clin, Sao Paulo, Brazil
关键词
Acid-etched surface; Healing caps; Machined surface; Peri-implant soft tissues; ENDOTHELIAL GROWTH-FACTOR; NITRIC-OXIDE SYNTHASE; DIFFERENT SURFACE-TOPOGRAPHY; PLAQUE-FORMATION; IN-VIVO; HUMAN GINGIVAL; ORAL IMPLANTS; EXPRESSION; ROUGHNESS; BONE;
D O I
10.1007/s00784-011-0574-3
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
A close spatial correlation has been described between the roughness of intraoral materials and the rate of bacterial colonisation. The aim of the present study in man was to conduct a comparative immunohistochemical evaluation of the inflammatory infiltrate, microvessel density, the nitric oxide synthases 1 and 3 and the vascular endothelial growth factor expression, the proliferative activity, and the B and T lymphocyte and histiocyte positivity in the peri-implant soft tissues around machined and acid-etched titanium healing caps. Ten patients participated in this study. The patients were enrolled consecutively. All patients received dental implants left to heal in a non-submerged mode. Healing caps were inserted in all implants. Half of the implants were supplied randomly with machined caps of titanium (control), while the other half were provided randomly with acid-etched titanium caps (test). After a 6-month healing period, a gingival biopsy was performed with a circular scalpel around the healing caps of both groups. The inflammatory infiltrate was mostly present in specimens. Their extension was much larger than that of the samples. A higher number of T and B lymphocytes were observed in specimens. Higher values of microvessel density and a higher expression of vascular endothelial growth factor intensity were observed in the samples. Furthermore, the Ki-67, NOS1 and NOS3 expression was significantly higher in the specimens. All these results showed that the tissues around healing caps underwent a higher rate of restorative processes, most probably correlated to the higher inflammation processes observed in these tissues.
引用
收藏
页码:857 / 866
页数:10
相关论文
共 44 条
[1]   Nitric oxide synthesis and severity of human periodontal disease [J].
Batista, AC ;
Silva, TA ;
Chun, JH ;
Lara, VS .
ORAL DISEASES, 2002, 8 (05) :254-260
[2]   The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis [J].
Bollen, CML ;
Papaioanno, W ;
VanEldere, J ;
Schepers, E ;
Quirynen, M ;
vanSteenberghe, D .
CLINICAL ORAL IMPLANTS RESEARCH, 1996, 7 (03) :201-211
[3]   Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: A review of the literature [J].
Bollen, CML ;
Lambrechts, P ;
Quirynen, M .
DENTAL MATERIALS, 1997, 13 (04) :258-269
[4]   The role of nitric oxide in oral diseases [J].
Brennan, PA ;
Thomas, GJ ;
Langdon, JD .
ARCHIVES OF ORAL BIOLOGY, 2003, 48 (02) :93-100
[5]   Human gingival fibroblasts produce nitric oxide in response to proinflammatory cytokines [J].
Daghigh, F ;
Borghaei, RC ;
Thornton, RD ;
Bee, JH .
JOURNAL OF PERIODONTOLOGY, 2002, 73 (04) :392-400
[6]   Biological factors contributing to failures of osseointegrated oral implants - (II). Etiopathogenesis [J].
Esposito, M ;
Hirsch, JM ;
Lekholm, U ;
Thomsen, P .
EUROPEAN JOURNAL OF ORAL SCIENCES, 1998, 106 (03) :721-764
[7]   Plaque formation on surface modified dental implants -: An in vitro study [J].
Grössner-Schreiber, B ;
Griepentrog, MH ;
Haustein, I ;
Müller, WD ;
Lange, KP ;
Briedigkeit, H ;
Göbel, UB .
CLINICAL ORAL IMPLANTS RESEARCH, 2001, 12 (06) :543-551
[8]  
Heydenrijk K, 2002, INT J ORAL MAX IMPL, V17, P829
[9]   Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue [J].
Hirose, M ;
Ishihara, K ;
Saito, A ;
Nakagawa, T ;
Yamada, S ;
Okuda, K .
JOURNAL OF PERIODONTOLOGY, 2001, 72 (05) :590-597
[10]   Leptin within healthy and diseased human gingiva [J].
Johnson, RB ;
Serio, FG .
JOURNAL OF PERIODONTOLOGY, 2001, 72 (09) :1254-1257