Surface modification to reduce nonspecific binding of quantum dots in live cell assays

被引：223

作者：

Bentzen, EL

Tomlinson, ID

Mason, J

Gresch, P

Warnement, MR

Wright, D

Sanders-Bush, E

Blakely, R

Rosenthal, SJ

机构：

[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN USA

来源：

BIOCONJUGATE CHEMISTRY | 2005年 / 16卷 / 06期

关键词：

D O I：

10.1021/bc0502006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonspecific binding is a frequently encountered problem with fluorescent labeling of tissue cultures when labeled with quantum dots. In these studies various cell lines were examined for nonspecific binding. Evidence suggests that nonspecific binding is related to cell type and may be significantly reduced by functionalizing quantum dots with poly(ethylene glycol) ligands (PEG). The length of PEG required to give a significant reduction in nonspecific binding may be as short as 12-14 ethylene glycol units.

引用

收藏

页码：1488 / 1494

页数：7

相关论文

共 29 条

[1] Nanocrystal targeting in vivo [J].

Åkerman, ME ;

Chan, WCW ;

Laakkonen, P ;

Bhatia, SN ;

Ruoslahti, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12617-12621

[2] Perspectives on the physical chemistry of semiconductor nanocrystals [J].

Alivisatos, AP .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (31) :13226-13239

[3] Noninvasive imaging of quantum dots in mice [J].

Ballou, B ;

Lagerholm, BC ;

Ernst, LA ;

Bruchez, MP ;

Waggoner, AS .

BIOCONJUGATE CHEMISTRY, 2004, 15 (01) :79-86

[4] Progression of respiratory syncytial virus infection monitored by fluorescent quantum dot probes [J].

Bentzen, EL ;

House, F ;

Utley, TJ ;

Crowe, JE ;

Wright, DW .

NANO LETTERS, 2005, 5 (04) :591-595

[5] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[6] Luminescent quantum dots for multiplexed biological detection and imaging [J].

Chan, WCW ;

Maxwell, DJ ;

Gao, XH ;

Bailey, RE ;

Han, MY ;

Nie, SM .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) :40-46

[7] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[8] Trichosanthin induced calcium-dependent generation of reactive oxygen species in human choriocarcinoma cells [J].

Chun-Yang, Z ;

Yi-Xuan, G ;

Hui, M ;

Cheng-Cai, A ;

Die-Yan, C .

ANALYST, 2000, 125 (09) :1539-1542

[9] (CdSe)ZnS core-shell quantum dots: Synthesis and characterization of a size series of highly luminescent nanocrystallites [J].

Dabbousi, BO ;

RodriguezViejo, J ;

Mikulec, FV ;

Heine, JR ;

Mattoussi, H ;

Ober, R ;

Jensen, KF ;

Bawendi, MG .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (46) :9463-9475

[10] In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].

Dubertret, B ;

Skourides, P ;

Norris, DJ ;

Noireaux, V ;

Brivanlou, AH ;

Libchaber, A .

SCIENCE, 2002, 298 (5599) :1759-1762