The Bcl-2 family pathway in gametes and preimplantation embryos

Apoptosis, a form of cell death by self-destruction, has been reported in gametes and preimplantation embryo,., both in vitro and in vivo Recent evidence suggests that cell death processes, whose control deserves to be elucidated, can impact embryo developmental competence. Moreover, quality of the gametes (particularly of the oocytes) is relevant not only for their survival rates but exert in influence during the early stages of embryo development Thus, the investigation of apoptosis-related genes and mechanisms in early embryos is crucial. BCL-2 family proteins, through balanced interactions between pro- and anti-death members, play a pivotal role in controlling cell life and death. In this article, we review the literature concerning the expression of Bcl-2 family members in gametes and early embryos Research results indicate that the various Bcl-2 subfamilies (pro- and anti-apoptotic "multidomain" family members and "BH3-only" death factors exhibit a dynamic expression pattern during male and female gamete differentiation and early embryo development While pro-apoptotic Bax protein plays a critical role in germ cell and early embryo degeneration. the relative importance of the prosurvival (Bcl-2, Bcl-xL, Bcl-w, Mcl-1) and "BH3-only" (Bim, Bad, Bik) members is not clear. Although information on expression patterns of Bcl-2 family transcripts and proteins is necessary, other elements such as transcriptional control (by environmental stimuli), subcellular localization and post-translational modifications should also be taken into account Aside from basic research, a better understanding of apoptosis-related proteins and mechanisms involved In gamete and embryo viability at the molecular level may provide new guides for diagnosis and therapeutic strategies (C) 2009 Elsevier Masson SAS All rights reserved