Continuous isomerization of D-fructose to D-mannose by immobilized Agrobacterium radiobacter cells

被引：18

作者：

Hirose, J ^{[1
]}

Kinoshita, Y ^{[1
]}

Fukuyama, S ^{[1
]}

Hayashi, S ^{[1
]}

Yokoi, H ^{[1
]}

Takasaki, Y ^{[1
]}

机构：

[1] Miyazaki Univ, Fac Engn, Dept Appl Chem, Miyazaki 8892192, Japan

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 04期

关键词：

Agrobacterium radiobacter; D-mannose; immobilization; mannose isomerase;

D O I：

10.1023/A:1022301725817

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

D-Fructose was isomerized to D-mannose using immobilized Agrobacterium radiobacter that produces a thermostable mannose isomerase. The cells were immobilized by adsorption on chitosan or by glutaraldehyde crosslinking in the presence of albumin. Optimum conditions for mannose isomerase activity were 60degreesC and pH 7.5. Continuous reaction at 55degreesC was achieved with immobilized cells packed in a column to produce D-mannose.

引用

页码：349 / 352

页数：4

共 8 条

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