Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences

被引：9
|
作者：
Molnar, Istvan [1 ]
Schneider, Annamaria [1 ]
Molnar-Lang, Marta [1 ]
机构：
[1] Hungarian Acad Sci, Agr Res Inst, POB 19, H-2462 Martonvasar, Hungary
来源：
CEREAL RESEARCH COMMUNICATIONS | 2005年 / 33卷 / 04期
关键词：
Aegilops biuncialis; Aegilops umbellulata; GISH; FISH; GAA banding;
D O I：
10.1556/CRC.33.2005.2-3.134
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The aims of the study were the optimisation of genomic in situ hybridization (GISH) and the use of a GAA satellite sequences as a fluorescence in situ hybridization (FISH) probe to discriminate Aegilops biuncialis and wheat chromosomes. The application of genomic DNA from the diploid progenitors, Ae. umbellulata and Ae. comosa, as a probe with a blocking ratio of 1:200 resulted in the clear visualization of Ae. biuncialis chromosomes. Based on the strong hybridization signals of the PCR-amplified GAA satellite sequences it was possible to identify chromosomes 1U, 2U, 4U and 5U of Ac. umbellulata. The improved GISH and GAA idiogram presented here allow the Ae. biuncialis chromatin to be traced in more detail during chromosome-mediated gene transfer to wheat.
引用
收藏
页码：673 / 680
页数：8
相关论文
共 50 条
  • [1] Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences
    István Molnár
    Annamária Schneider
    Márta Molnár-Láng
    Cereal Research Communications, 2005, 33 : 673 - 680
  • [2] GISH reveals different levels of meiotic pairing with wheat for individual Aegilops biuncialis chromosomes
    Molnar, I.
    Molnar-Lang, M.
    BIOLOGIA PLANTARUM, 2010, 54 (02) : 259 - 264
  • [3] Detection of Various U and M Chromosomes in Wheat-Aegilops biuncialis Hybrids and Derivatives Using Fluorescence in situ Hybridisation and Molecular Markers
    Schneider, Annamaria
    Molnar-Lang, Marta
    CZECH JOURNAL OF GENETICS AND PLANT BREEDING, 2012, 48 (04) : 169 - 177
  • [4] Evolutionary dynamics and chromosomal distribution of repetitive sequences on chromosomes of Aegilops speltoides revealed by genomic in situ hybridization
    Belyayev, A
    Raskina, O
    Nevo, E
    HEREDITY, 2001, 86 : 738 - 742
  • [5] Evolutionary dynamics and chromosomal distribution of repetitive sequences on chromosomes of Aegilops speltoides revealed by genomic in situ hybridization
    Alexander Belyayev
    Olga Raskina
    Eviatar Nevo
    Heredity, 2001, 86 (6) : 738 - 742
  • [6] Identification of parental chromosomes in hybridogenetic water frog Pelophylax esculentus (Rana esculenta) by genomic in situ hybridization (GISH)
    Zalesna, A.
    Choleva, L.
    Ogielska, M.
    Rabova, M.
    Marec, F.
    Rab, P.
    CHROMOSOME RESEARCH, 2010, 18 (06) : 754 - 755
  • [7] Identification of the parental chromosomes of the wheat-alien amphiploid Agrotana by genomic in situ hybridization
    Chen, Q
    Conner, RL
    Laroche, A
    GENOME, 1995, 38 (06) : 1163 - 1169
  • [8] Identification of wheat and tritordeum chromosomes by genomic in situ hybridization using total Hordeum chilense DNA as probe
    González, MJ
    Cabrera, A
    GENOME, 1999, 42 (06) : 1194 - 1200
  • [9] Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp mays with B chromosomes
    Poggio, L
    Confalonieri, V
    Comas, C
    Cuadrado, A
    Jouve, N
    Naranjo, CA
    GENOME, 1999, 42 (04) : 687 - 691
  • [10] Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids
    Cui, Litao
    Chen, Tai
    Zhao, Xin
    Wang, Shunli
    Ren, Xiuxia
    Xue, Jingqi
    Zhang, Xiuxin
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (19)
12345