Immunohistochemical expression of Epidermal Growth Factor Receptor (EGFR) in canine mammary tissues

被引：65
作者：
Gama, A. [2 ]
Gaertner, F. [1 ,3 ]
Alves, A. [2 ]
Schmitt, F. [1 ,4 ]
机构：
[1] Univ Porto IPATIMUP, Inst Mol Pathol & Immunol, P-4200465 Oporto, Portugal
[2] Univ Tras os Montes & Alto Douro UTAD, CECAV, Dept Vet Sci, P-5001811 Vila Real, Portugal
[3] Univ Porto, Inst Ciencias Biomed Abel Salazar, P-4099003 Oporto, Portugal
[4] Univ Porto, Fac Med, P-4100 Oporto, Portugal
来源：
RESEARCH IN VETERINARY SCIENCE | 2009年 / 87卷 / 03期
关键词：
Canine; Mammary tumours; EGFR; Immunohistochemistry; BREAST-CANCER; PROTEIN EXPRESSION; PROGNOSTIC VALUE; MESSENGER-RNA; GENE; OVEREXPRESSION; AMPLIFICATIONS; CARCINOMAS; BENIGN; MARKER;
D O I：
10.1016/j.rvsc.2009.04.016
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Epidermal Growth Factor Receptor (EGFR) has been extensively studied in human breast cancer; however, systematic studies of EGFR protein expression in canine mammary gland tumours are lacking. Therefore, we evaluated its immunohistochemical expression in a series of 136 canine mammary tumours and representative areas of adjacent normal and hyperplastic mammary tissue and investigated a possible correlation between EGFR overexpression and several clinicopathological parameters and survival. In normal and hyperplastic canine mammary glands, EGFR expression was consistently observed in myoepithelial cells, with luminal cells usually negative. In tumour tissues, EGFR overexpression was found in 9 benign (19.6%) and 38 malignant (42.2%) lesions, with EGFR positivity significantly related with malignancy. Besides animal age and tumour size, there were no significant associations between other clinicopathological parameters and EGFR overexpression. On survival analysis, tumours with EGFR overexpression showed a reduced disease-free and overall survival; however these associations failed to reach statistically significant levels. (c) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：432 / 437
页数：6
相关论文
共 37 条
[1]   Prognostic relevance of gene amplifications and coamplifications in breast cancer [J].
Al-Kuraya, K ;
Schraml, P ;
Torhorst, J ;
Tapia, C ;
Zaharieva, B ;
Novotny, H ;
Spichtin, H ;
Maurer, R ;
Mirlacher, M ;
Köchli, O ;
Zuber, M ;
Dieterich, H ;
Mross, F ;
Wilber, K ;
Simon, R ;
Sauter, G .
CANCER RESEARCH, 2004, 64 (23) :8534-8540
[2]   Critical update and emerging trends in epidermal growth factor receptor targeting in cancer [J].
Baselga, J ;
Arteaga, CL .
JOURNAL OF CLINICAL ONCOLOGY, 2005, 23 (11) :2445-2459
[3]   EGFR gene amplification in breast cancer:: correlation with epidermal growth factor receptor mRNA and protein expression and HER-2 status and absence of EGFR-activating mutations [J].
Bhargava, R ;
Gerald, WL ;
Li, AR ;
Pan, QL ;
Lal, P ;
Ladanyi, M ;
Chen, BY .
MODERN PATHOLOGY, 2005, 18 (08) :1027-1033
[4]   EPIDERMAL GROWTH-FACTOR [J].
CARPENTER, G ;
COHEN, S .
ANNUAL REVIEW OF BIOCHEMISTRY, 1979, 48 :193-216
[5]   Epidermal growth factor receptor (EGFR) as a target in cancer therapy: understanding the role of receptor expression and other molecular determinants that could influence the response to anti-EGFR drugs [J].
Ciardiello, F ;
Tortora, G .
EUROPEAN JOURNAL OF CANCER, 2003, 39 (10) :1348-1354
[6]   Immunolocalization of the smooth muscle-specific protein calponin in complex and mixed tumors of the mammary gland of the dog:: Assessment of the morphogenetic role of the myoepithelium [J].
de los Monteros, AE ;
Millán, MY ;
Ordás, J ;
Carrasco, L ;
Reymundo, C ;
de las Mulas, JM .
VETERINARY PATHOLOGY, 2002, 39 (02) :247-256
[7]   IMMUNOHISTOCHEMICAL IDENTIFICATION OF MYOEPITHELIAL, EPITHELIAL, AND CONNECTIVE-TISSUE CELLS IN CANINE MAMMARY-TUMORS [J].
DESTEXHE, E ;
LESPAGNARD, L ;
DEGEYTER, M ;
HEYMANN, R ;
COIGNOUL, F .
VETERINARY PATHOLOGY, 1993, 30 (02) :146-154
[8]  
DONNAY I, 1993, J REPROD FERTIL, P501
[9]   Relationship between receptors for epidermal growth factor and steroid hormones in normal, dysplastic and neoplastic canine mammary tissues [J].
Donnay, I ;
Devleeschouwer, N ;
WoutersBallman, P ;
Leclercq, G ;
Verstegen, J .
RESEARCH IN VETERINARY SCIENCE, 1996, 60 (03) :251-254
[10]  
Elston C W., 1998, Systemic pathology: the Breast, V13, P365
1234