One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography

被引:47
作者
Gu, Ming [1 ]
Wang, Xiaolei [1 ]
Su, Zhiguo [1 ]
Fan Ouyang [1 ]
机构
[1] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
关键词
high-speed counter-current chromatography; polyphenol; 3,4-dihydroxyphenyllactic acid; salvianolic acid B; protocatechualdehyde;
D O I
10.1016/j.chroma.2006.11.057
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Three kinds of polyphenols of Salvia miltiorrhiza Bunge, 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde, were separated and purified in one step with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:6:1.5:1.5:8) by high-speed countercurrent chromatography. Acetic acid was successfully used to increase the partition of high polar target compounds in organic phase to modify partition coefficient value. 3,4-Dihydroxyphenyl lactic acid, salvianolic acid B and protocatechualdehyde were purified from 100 mg water extracted crude sample of Salvia miltiorrhiza Bunge at purity of 97.6%, 94.2% and 98.2% and at yield of 98.6%, 73.6% and 90.2%. High-speed countercurrent chromatography together with organic/aqueous solvent system supplied an efficient method to purify water-soluble compounds directly from crude samples of traditional Chinese medicines. (c) 2006 Elsevier B.V All rights reserved.
引用
收藏
页码:107 / 111
页数:5
相关论文
共 18 条
[1]   Separation and identification of water-soluble salvianolic acids from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography and ESI-MS analysis [J].
Chen, JH ;
Wang, FM ;
Lee, FSC ;
Wang, XR ;
Xie, MY .
TALANTA, 2006, 69 (01) :172-179
[2]   How to achieve rapid separations in counter-current chromatography [J].
Chen, LJ ;
Sutherland, IA .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1114 (01) :29-33
[3]   Comparison of high-speed counter-current chromatography and high-performance liquid chromatography on fingerprinting of Chinese traditional medicine [J].
Gu, M ;
Fan, OY ;
Su, ZG .
JOURNAL OF CHROMATOGRAPHY A, 2004, 1022 (1-2) :139-144
[4]   Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography [J].
Ito, Y .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1065 (02) :145-168
[5]   RECENT ADVANCES IN COUNTERCURRENT CHROMATOGRAPHY [J].
ITO, Y .
JOURNAL OF CHROMATOGRAPHY, 1991, 538 (01) :3-25
[6]   Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography [J].
Li, HB ;
Lai, JP ;
Jiang, Y ;
Chen, F .
JOURNAL OF CHROMATOGRAPHY A, 2002, 943 (02) :235-239
[7]   Preparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography [J].
Li, HB ;
Chen, F .
JOURNAL OF CHROMATOGRAPHY A, 2001, 925 (1-2) :109-114
[8]   Diterpenoids from Salvia miltiorrhiza [J].
Lin, HC ;
Chang, WL .
PHYTOCHEMISTRY, 2000, 53 (08) :951-953
[9]   Salvianolic acid B, an antioxidant from Salvia miltiorrhiza, prevents Aβ25-35-induced reduction in BPRP in PC12 cells [J].
Lin, Yan-Hua ;
Liu, Ai-Hua ;
Wu, Hong-Li ;
Westenbroek, Christel ;
Song, Qian-Liu ;
Yu, He-Ming ;
Ter Horst, Gert J. ;
Li, Xue-Jun .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 348 (02) :593-599
[10]   Polyphenolics of Salvia -: a review [J].
Lu, YR ;
Foo, LY .
PHYTOCHEMISTRY, 2002, 59 (02) :117-140