Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside

被引：7

作者：

Horinouchi, Nobuyuki ^{[1
]}

Kawano, Takako ^{[1
]}

Sakai, Takafumi ^{[1
]}

Matsumoto, Seiichiro ^{[2
]}

Sasaki, Mie ^{[2
]}

Mikami, Yoichi ^{[2
]}

Ogawa, Jun ^{[1
,3
]}

Shimizu, Sakayu ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Sci, Sakyo Ku, Kyoto 6068502, Japan

[2] Yuki Gosei Kogyo Ltd, Tokyo Lab, Itabashi Ku, Tokyo 1740043, Japan

[3] Kyoto Univ, Res Div Microbial Sci, Sakyo Ku, Kyoto 6068502, Japan

来源：

NEW BIOTECHNOLOGY | 2009年 / 26卷 / 1-2期

关键词：

ESCHERICHIA-COLI K-12; DEOXYRIBONUCLEOSIDE CATABOLISM; 2-DEOXYRIBOSE; 5-PHOSPHATE; DEOXYRIBOALDOLASE; ACETALDEHYDE; GLUCOSE; RIBONUCLEOSIDE; REDUCTASE; ALDOLASE; MUTANTS;

D O I：

10.1016/j.nbt.2009.03.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2'-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and D-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the practical reaction mixture for enzymatic 2'-deoxyribonucleoside production.

引用

页码：75 / 82

页数：8

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