Aetiology of Opuntia ficus-indica malformations and stunting disease

被引:13
作者
Granata, G.
Paltrinieri, S.
Botti, S.
Bertaccini, A.
机构
[1] Univ Bologna, DISTA, Alma Mater Studiorum, I-40127 Bologna, Italy
[2] Univ Catania, DISTEF Patol Vegetale, Catania, Italy
关键词
cactus pear; disease aetiology; molecular identification; phytoplasma;
D O I
10.1111/j.1744-7348.2006.00097.x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The phytoplasma aetiology of epidemic stunting of cladodes and stunted growth observed in a cactus pear plantation in Carlentini (Sicily, Italy) was investigated with graft inoculation trials and PCR/restriction fragment length polymorphism analyses of 16S ribosomal RNA (rRNA) gene and S3 ribosomal protein gene. After sequencing of the 1525-bp from 16S rRNA gene of both naturally infected and graft-inoculated symptomatic cactus pear samples, phytoplasma TS belonging to ribosomal subgroup 16SrII-C were identified as aetiological agents of this worldwide spread disease.
引用
收藏
页码:317 / 325
页数:9
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