Synthesis of an artificial glycoconjugate polymer carrying Pk-antigenic trisaccharide and its potent neutralization activity against Shiga-like toxin

被引：79

作者：

Dohi, H

Nishida, Y

Mizuno, M

Shinkai, M

Kobayashi, T

Takeda, T

Uzawa, H

Kobayashi, K ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Dept Mol Design & Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan

[2] Nagoya Univ, Grad Sch Engn, Dept Biotechnol, Chikusa Ku, Nagoya, Aichi 4648603, Japan

[3] Natl Childrens Med Res Ctr, Dept Infect Dis Res, Setagaya Ku, Tokyo 154, Japan

[4] Natl Inst Mat & Chem Res, Tsukuba, Ibaraki 3058565, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1999年 / 7卷 / 09期

关键词：

D O I：

10.1016/S0968-0896(99)00129-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence-labeled glycoconjugate polymers carrying carbohydrate segments of a globotriaosyl ceramide (Gb(3)) were synthesized and subjected to biological assays using Escherichia coli O-157 strains and Shiga-like toxins (Stx-I and Stx-II). For the fluorescence labeling, a new polymerizable fluorescent monomer with a TBMB carbonyl chromophore (Ex. 325 nm, Em. 410 nm) was designed. A glycosyl monomer of the trisaccharide segment of Gb(3) was prepared from p-nitrophenyl beta-lactoside and copolymerized with acrylamide and the fluorescent monomer to prepare a fluorescence-labeled glycoconjugate copolymer carrying [alpha-D-galactopyranosyl-(1-->4)-beta-D-galactopyranosyl]-(1-->4)- beta-D-glucopyranoside. The polymer showed potent neutralization activity against Stx-I and also binding activity onto E. coli O-157 strains. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：2053 / 2062

页数：10

共 30 条

[1] Influence of phospholipid chain length on verotoxin/globotriaosyl ceramide binding in model membranes: Comparison of a supported bilayer film and liposomes [J].