Synthesis of an artificial glycoconjugate polymer carrying Pk-antigenic trisaccharide and its potent neutralization activity against Shiga-like toxin

被引：79
作者：
Dohi, H
Nishida, Y
Mizuno, M
Shinkai, M
Kobayashi, T
Takeda, T
Uzawa, H
Kobayashi, K [1 ]
机构：
[1] Nagoya Univ, Grad Sch Engn, Dept Mol Design & Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan
[2] Nagoya Univ, Grad Sch Engn, Dept Biotechnol, Chikusa Ku, Nagoya, Aichi 4648603, Japan
[3] Natl Childrens Med Res Ctr, Dept Infect Dis Res, Setagaya Ku, Tokyo 154, Japan
[4] Natl Inst Mat & Chem Res, Tsukuba, Ibaraki 3058565, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 1999年 / 7卷 / 09期
关键词：
D O I：
10.1016/S0968-0896(99)00129-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorescence-labeled glycoconjugate polymers carrying carbohydrate segments of a globotriaosyl ceramide (Gb(3)) were synthesized and subjected to biological assays using Escherichia coli O-157 strains and Shiga-like toxins (Stx-I and Stx-II). For the fluorescence labeling, a new polymerizable fluorescent monomer with a TBMB carbonyl chromophore (Ex. 325 nm, Em. 410 nm) was designed. A glycosyl monomer of the trisaccharide segment of Gb(3) was prepared from p-nitrophenyl beta-lactoside and copolymerized with acrylamide and the fluorescent monomer to prepare a fluorescence-labeled glycoconjugate copolymer carrying [alpha-D-galactopyranosyl-(1-->4)-beta-D-galactopyranosyl]-(1-->4)- beta-D-glucopyranoside. The polymer showed potent neutralization activity against Stx-I and also binding activity onto E. coli O-157 strains. (C) 1999 Elsevier Science Ltd. All rights reserved.
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页码：2053 / 2062
页数：10
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