Real-time analysis of membrane permeabilizing effects of oleanane saponins

被引：48
作者：
Gilabert-Oriol, Roger [1 ]
Mergel, Katharina [1 ]
Thakur, Mayank [1 ]
von Mallinckrodt, Benedicta [1 ]
Melzig, Matthias F. [2 ]
Fuchs, Hendrik [1 ]
Weng, Alexander [1 ]
机构：
[1] Charite, Inst Lab Med Klin Chem & Pathobiochem, D-12200 Berlin, Germany
[2] Free Univ Berlin, Inst Pharm Pharmazeut Biol, D-14195 Berlin, Germany
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2013年 / 21卷 / 08期
关键词：
Real-time analysis; Biomembranes; Permeabilizing effects; Electrophoresis; Saponin; TARGETED TOXINS; GYPSOPHILA SAPONINS; CYTOTOXICITY; CELLS; ALBUM; ENHANCEMENT; STIMULATION; CHOLESTEROL; ADJUVANTS; DIGITONIN;
D O I：
10.1016/j.bmc.2013.01.061
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Saponins are a group of plant and marine derived glycosides with numerous biological functions. Two important characteristics of certain plant saponins are their ability to enhance cytotoxicity of type I ribosome inactivating proteins and stimulation of the immune system. The main objective of the present study was to investigate in real-time the permeabilizing effects of saponins on cell membrane. A set of oleanane saponins (glycyrrhizinic acid, Gypsophila, Saponaria and Quillaja saponins) and a steroid saponin (digitonin) were tested. The effects of these saponins on lysosomal membranes and hemolysis, along with their charge were also studied. Real-time monitoring of cell membrane permeabilization facilitated a highly sensitive analysis of the cellular kinetics. Saponins showed variable permeabilizing effects on cellular and lysosomal membranes at concentrations from 6 mu M and hemolysis from 3 mu M. Further, the results suggest that charge of the saponin may be relevant for permeabilizing effects of oleanane saponins. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2387 / 2395
页数：9
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