Molecular characterization of a new synthetic cry2ab gene in Nicotiana tabacum

被引：1
作者：
Karimi, Sohail Mehmood [1 ,2 ]
Sohail, Muhammad Nouman [1 ]
Amin, Imran [1 ]
Mansoor, Shahid [1 ,2 ]
Mukhtar, Zahid [1 ]
机构：
[1] Natl Inst Biotechnol & Genet Engn, Faisalabad, Pakistan
[2] Univ Lahore, IMBB, Lahore, Pakistan
来源：
BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 06期
关键词：
Armyworms; Biocontrol; Biopesticides; Insect bioassay; Synthetic cry2Ab gene; BACILLUS-THURINGIENSIS; EXPRESSION; TOBACCO; RESISTANCE; CRY1AC; PLANTS;
D O I：
10.1007/s10529-013-1153-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A newly-synthesized cry2Ab gene was characterized in Nicotiana tabacum, before its further transformation in cotton. Synthetic cry2Ab gene was cloned in pGreen0029 and its expression was transiently analyzed at mRNA level through agroinfiltration in tobacco. The mRNA of cry2Ab was detected after 72 h agroinfiltration through PCR using total plant RNA. This construct was then transformed into N. tabacum through Agrobacterium. Insect bioassays were conducted on detached leaves using first instar Spodoptera exigua larvae; after 96 h significant insect mortality was recorded. This newly synthesized gene was effective in controlling S. exigua first instar larvae. It can be used in combinations with other Bt genes like cry1Ac for developing resistance against major insect pests of cotton and further widening the insect control spectrum.
引用
收藏
页码：969 / 974
页数：6
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