Interaction effects of mesoporous silica nanoparticles with different morphologies on human red blood cells

被引：40
作者：
Joglekar, Madhura [1 ]
Roggers, Robert A. [2 ]
Zhao, Yannan [3 ]
Trewyn, Brian G. [1 ]
机构：
[1] Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA
[2] Wayne State Univ, Dept Pharmaceut Sci, Detroit, MI USA
[3] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
来源：
RSC ADVANCES | 2013年 / 3卷 / 07期
基金：
美国国家科学基金会;
关键词：
RECEPTOR-MEDIATED ENDOCYTOSIS; INTRACELLULAR DELIVERY; CELLULAR UPTAKE; SHAPE; MEMBRANE; SIZE; FUNCTIONALIZATION; PARTICLES; DESIGN; MECHANISM;
D O I：
10.1039/c2ra22264g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A series of MCM-41-type mesoporous silica nanoparticle (MSN) materials with different morphologies (spherical and tubular) and sizes but similar surface and pore properties were synthesized and characterized. The effect of particle morphology on human red blood cell (RBC) membrane integrity was evaluated using flow cytometry, UV-Vis spectroscopy, confocal fluorescence and electron microscopy. Although the hemolytic activity was miniscule at higher nanoparticle concentrations for MSN with spherical geometry (up to 500 mu g mL(-1)), electron microscopy results showed significant occurrences of RBC spiculation, indicating damage to the plasma membrane. However, no evidence for particle embedment or internalization was found in all the geometries and particle concentrations tested. We envisage that complete hemocompatibility studies of varied nanoparticle morphologies would provide important guidelines when considering the overall toxicity of these nanodevices in vivo.
引用
收藏
页码：2454 / 2461
页数：8
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