The effect of Notch signal pathway on PM2.5-induced Muc5ac in Beas-2B cells

被引：14

作者：

Liu, Ying ^{[1
]}

Zhou, Tong ^{[2
]}

Sun, Luyao ^{[3
]}

Wang, Haodong ^{[4
]}

Zhou, Liting ^{[1
,5
]}

机构：

[1] Jilin Univ, First Hosp Jilin Univ, Dept Resp Med, Changchun, Peoples R China

[2] Jilin Univ, First Hosp Jilin Univ, Dept Endocrinol, Changchun, Peoples R China

[3] Jilin Univ, First Hosp Jilin Univ, Dept Infect Dis, Changchun, Peoples R China

[4] Jilin Univ, First Hosp Jilin Univ, Dept Vasc Surg, Changchun, Peoples R China

[5] Jilin Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Changchun, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2020年 / 203卷

基金：

中国国家自然科学基金;

关键词：

Notch signaling pathway; Muc5ac; PM2.5; Beas-2B cells; AIR-POLLUTION; EXPRESSION; MUCUS; METAPLASIA; EPITHELIUM; BURDEN;

D O I：

10.1016/j.ecoenv.2020.110956

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Background: Atmospheric pollutants could induced over-expression of Muc5ac, which is a major pathological feature in acute exacerbation of Chronic Obstructive Pulmonary Disease (COPD) and fatal asthma. Notch signaling pathway could promote mucus cell proliferation and mucus secretion. However, the effects of Notch signaling pathway on the airway mucus secretion induced by PM2.5 remain unknown. In this study, we investigated the role of the Notch signaling pathway on Muc5ac by atmospheric PM2.5 in Beas-2B cell. Methods: The mRNA and protein levels of the Notchl-4, downstream target gene Hesl and Muc5ac in the Notch signaling pathway were detected by qPCR and western after Beas-2B cells were exposed to PM2.5 of different concentrations for 12h, 24h, and 48h. Results: The longer the exposure time and the higher the concentration of PM2.5 , the lower the survival rate of Beas-2B cells. The expressions of Hesl and Muc5ac in mRNA and protein were significantly increased after PM2.5 exposure. Correlation analysis indicated that there was a positive correlation between the expression of Muc5ac and Hesl in mRNA and protein. Conclusion: Atmospheric PM2.5 can induce the express of Muc5ac, the Notch signaling pathway may be involved in the regulation of Muc5ac by Hes1.

引用

页数：11