Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis

被引:7
作者
Reddy, Ashok Kumar
Brahmaiah, Upputuri
Narayen, Nitesh
Reddy, Ravi Kumar
Reddy, Rupak Kumar
Chitta, Meghraj
Prasad, Srinivas
Swarup, Rishi
Mohiuddin, Syed Maaz
Reddy, Madhukar
Aasuri, Murali K.
Murthy, B. S. R.
Bhide, Milind
Ahmed, Sajid
机构
[1] Department of Microbiology, GHR Micro Diagnostics, Punjagutta, Hyderabad, Dwarakapuri Colony
[2] M.N.R Medical College, Sangareddy
[3] Maxivision Eye Centre, Hyderabad
[4] Medivision Eye and Health Care Centre, Hyderabad
[5] Sarojini Devi Eye Hospital, Hyderabad
[6] Swarup Eye Centre, Hyderabad
[7] Eye Care Hyderabad, Hyderabad
[8] Drishti Eye Centre, Hyderabad
[9] Sulochana Eye Centre, Hyderabad
[10] Vasan Eye Care, Hyderabad
[11] Hyderabad Eye Centre, Hyderabad
[12] Arvind Eye Hospital, Hyderabad
[13] Neo Retina, Hyderabad
关键词
EYE CARE CENTER; MICROBIOLOGICAL DIAGNOSIS; EPIDEMIOLOGIC FEATURES; SOUTH-INDIA;
D O I
10.1007/s10792-012-9683-5
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
To compare the blood agar (BA), sabouraud dextrose agar (SDA) and chocolate agar (CA) for the isolation of fungi in patients with mycotic keratitis. Corneal Scrapings of 229 patients with clinically diagnosed microbial keratitis were inoculated on BA, SDA, CA. The culture media were evaluated for the rate and time taken for the fungal growth. Seventy six of 229 patients had fungal keratitis. Fungus grew on BA in 60/76(78.9 %), on SDA in 76/76 (100 %), on CA in 40/76(52.6 %) patients. The fungi which grew on BA (60/76) also grown on SDA at the same time. The colony morphologies of different fungi were better on SDA than BA/CA. Among the different culture media, SDA is essential for the isolation fungi in patients with mycotic keratitis.
引用
收藏
页码:251 / 254
页数:4
相关论文
共 10 条
  • [1] Microbiological diagnosis of infective keratitis: comparative evaluation of direct microscopy and culture results
    Bharathi, M. J.
    Ramakrishnan, R.
    Meenakshi, R.
    Mittal, S.
    Shivakumar, C.
    Srinivasan, M.
    [J]. BRITISH JOURNAL OF OPHTHALMOLOGY, 2006, 90 (10) : 1271 - 1276
  • [2] Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
    Das, Sujata
    Sharma, Savitri
    Kar, Sarita
    Sahu, Srikant K.
    Samal, Bikash
    Mallick, Aparajita
    [J]. INDIAN JOURNAL OF OPHTHALMOLOGY, 2010, 58 (04) : 281 - 285
  • [3] The epidemiological features and laboratory results of fungal keratitis - A 10-year review at a referral eye care center in south India
    Gopinathan, U
    Garg, P
    Fernandes, M
    Sharma, S
    Athmanathan, S
    Rao, GN
    [J]. CORNEA, 2002, 21 (06) : 555 - 559
  • [4] Review of epidemiological features, microbiological diagnosis and treatment outcome of microbial keratitis: Experience of over a decade
    Gopinathan, Usha
    Sharma, Savitri
    Garg, Prashant
    Rao, Gullapalli N.
    [J]. INDIAN JOURNAL OF OPHTHALMOLOGY, 2009, 57 (04) : 273 - 279
  • [5] Diagnosis and Treatment Outcome of Mycotic Keratitis at a Tertiary Eye Care Center in Eastern India
    Rautaraya, Bibhudutta
    Sharma, Savitri
    Kar, Sarita
    Das, Sujata
    Sahu, Srikant K.
    [J]. BMC OPHTHALMOLOGY, 2011, 11
  • [6] Srinivasan M, 2004, Curr Opin Ophthalmol, V15, P321, DOI 10.1097/00055735-200408000-00008
  • [7] Epidemiology and aetiological diagnosis of corneal ulceration in Madurai, south India
    Srinivasan, M
    Gonzales, CA
    George, C
    Cevallos, V
    Mascarenhas, JM
    Asokan, B
    Wilkins, J
    Smolin, G
    Whitcher, JP
    [J]. BRITISH JOURNAL OF OPHTHALMOLOGY, 1997, 81 (11) : 965 - 971
  • [8] Fungal infections of the cornea
    Thomas, PA
    [J]. EYE, 2003, 17 (08) : 852 - 862
  • [9] Current perspectives on ophthalmic mycoses
    Thomas, PA
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2003, 16 (04) : 730 - +
  • [10] Single culture media in infectious keratitis
    Waxman, E
    Chechelnitsky, M
    Mannis, MJ
    Schwab, IR
    [J]. CORNEA, 1999, 18 (03) : 257 - 261