Molecular cloning and characterization of the polypeptide backbone of Schistosoma mansoni circulating cathodic antigen

被引：2

作者：

Abdeen, HH ^{[1
]}

Attallah, AFM ^{[1
]}

Mansour, MM ^{[1
]}

Harrison, RA ^{[1
]}

机构：

[1] USN, Med Res Unit 3, Cairo, Egypt

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1999年 / 101卷 / 1-2期

关键词：

Schistosoma mansoni; Schistosoma haematobium; circulating cathodic antigen; diagnosis; cloning and expression;

D O I：

10.1016/S0166-6851(99)00068-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the candidate schistosome antigens for the development of a circulating antigen detection diagnostic assay is the circulating cathodic antigen (CCA). Detection of CCA in urine provides a non-invasive assay with high sensitivity. Previously we reported that CCA is secreted in patients' urine as a small molecular weight material which is probably of a proteinaceous nature. In an attempt to further characterize the secreted component of CCA, we used a monoclonal antibody (MAb) reactive with urine-CCA to isolate an adult worm cDNA clone (SmN3-1) that encodes the polypeptide backbone of CCA. The sequence, gene organization and expression of SmN3-1 were analyzed. The 1.6 kb nucleotide and 347 amino acid sequences of SmN3-1 showed no significant homology to any published sequence. The size and antigenic properties of the expression product of SmN3-1 in Escherichia coli greatly resembled the CCA molecule excreted in urine, suggesting that the latter is primarily composed of the protein element of CCA. (C) 1999 Published by Elsevier Science B.V.

引用

页码：149 / 159

页数：11

共 33 条

[1] Schistosoma mansoni:: The circulating cathodic antigen forms an abundant product of 41/42 kDa in the urine of infected patients [J].