Adsorption of cellulase on poly(2-hydroxyethyl methacrylate) cryogels containing phenylalanine

被引：5
|
作者：
Yavuz, Murat [1 ]
Cakir, Oguz [2 ]
Baysal, Zubeyde [1 ]
机构：
[1] Dicle Univ, Dept Chem, Fac Sci, Diyarbakir, Turkey
[2] Dicle Univ, Sci & Technol Applicat & Res Ctr DUBTAM, Diyarbakir, Turkey
来源：
TURKISH JOURNAL OF CHEMISTRY | 2016年 / 40卷 / 05期
关键词：
Cellulase; adsorption; cryogel; P(HEMA-MAPA); COMPOSITE CRYOGELS; LYSOZYME PURIFICATION; ASPERGILLUS-NIGER; IMMOBILIZATION; AFFINITY; MEMBRANES; CHROMATOGRAPHY; HYDROLYSIS; EFFICIENCY; HYDROGELS;
D O I：
10.3906/kim-1601-43
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The aim of this study was to prepare a supermacroporous cryogel that can be used for the adsorption of cellulase. The macroporous cryogel of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-phenylalanine) [p(HEMA-MAPA)] was prepared by copolymerization of 2-hydroxyethyl methacrylate (HEMA) with a functional monomer of N-methacryloyl-L-phenylalanine (MAPA). The cryogel was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and swelling tests. The effects of several parameters such as medium pH, temperature, ionic strength, and flow rate on cellulase adsorption were also investigated. Maximum cellulase adsorption was observed at 25 degrees C and pH 4.0. Furthermore, adsorbed cellulase was desorbed from the cryogel by using 1.0 M NaCl. The p(HEMA-MAPA) cryogel could be used many times without the cellulase adsorption capacity decreasing significantly.
引用
收藏
页码：720 / 728
页数：9
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