Sperm DNA damage has a negative association with live-birth rates after IVF

被引：153
作者：
Simon, L. [1 ]
Proutski, I. [1 ]
Stevenson, M. [1 ]
Jennings, D. [2 ]
McManus, J. [2 ]
Lutton, D. [2 ]
Lewis, S. E. M. [1 ]
机构：
[1] Queens Univ Belfast, Inst Clin Sci, Ctr Publ Hlth, Belfast BT12 6BJ, Antrim, North Ireland
[2] Royal Jubilee Matern Serv, Reg Fertil Ctr, Belfast BT12 6BA, Antrim, North Ireland
来源：
REPRODUCTIVE BIOMEDICINE ONLINE | 2013年 / 26卷 / 01期
关键词：
Comet assay; ICSI; IVF; male infertility; sperm DNA damage; IN-VITRO FERTILIZATION; ASSISTED REPRODUCTIVE TECHNOLOGY; EMBRYO QUALITY; STRAND BREAKS; INTEGRITY ASSESSMENT; NORMAL SPERMATOZOA; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; PROGNOSTIC TOOL; PREGNANCY RATES;
D O I：
10.1016/j.rbmo.2012.09.019
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Sperm DNA damage has a negative impact on pregnancy rates following assisted reproduction treatment (ART). The aim of the present study was to examine the relationship between sperm DNA fragmentation and live-birth rates after IVF and intracytoplasmic sperm injection (ICSI). The alkaline Comet assay was employed to measure sperm DNA fragmentation in native semen and in spermatozoa following density-gradient centrifugation in semen samples from 203 couples undergoing IVF and 136 couples undergoing ICSI. Men were divided into groups according to sperm DNA damage. Following IVF, couples with <25% sperm DNA fragmentation had a live-birth rate of 33%; in contrast, couples with >50% sperm DNA fragmentation had a much lower live-birth rate of 13%. Following ICSI, no significant differences in sperm DNA damage were found between any groups of patients. Sperm DNA damage was also associated with low live-birth rates following IVF in both men and couples with idiopathic infertility: 39% of couples and 41% of men with idiopathic infertility have high sperm DNA damage. Sperm DNA damage assessed by the Comet assay has a close inverse relationship with live-birth rates after IVF. RBMOnline (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：68 / 78
页数：11
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