Ternatin, a cyclic peptide isolated from mushroom, and its derivative suppress hyperglycemia and hepatic fatty acid synthesis in spontaneously diabetic KK-Ay mice

被引：16

作者：

Kobayashi, Misato ^{[1
]}

Kawashima, Haruna ^{[1
]}

Takemori, Kumiko ^{[2
]}

Ito, Hiroyuki ^{[3
]}

Murai, Atsushi ^{[1
]}

Masuda, Shun ^{[4
]}

Yamada, Kaoru ^{[4
]}

Uemura, Daisuke ^{[4
]}

Horio, Fumihiko ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Biosci, Nagoya, Aichi 4648601, Japan

[2] Kinki Univ, Fac Agr, Dept Food & Nutr, Nara 6318505, Japan

[3] Kinki Univ, Dept Biomed Engn, Fac Biol Oriented Engn, Wakayama 6496493, Japan

[4] Kanagawa Univ, Dept Chem, Kanagawa 2591293, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 427卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

Ternatin; Cyclic peptide; Anti-diabetic; Blood glucose; Triglyceride; KK-A(y) mice; INSULIN-RESISTANCE; (-)-TERNATIN; ACCUMULATION; OBESITY; GINSENG; BERRY; PREVENTION; EXTRACT;

D O I：

10.1016/j.bbrc.2012.09.045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(-)-Ternatin is a highly methylated cyclic heptapeptide isolated from mushroom Coriolus versicolor. Ternatin has an inhibitory effect on fat accumulation in 3T3-L1 adipocytes. [D-Leu(7)]ternatin, a ternatin derivative, also inhibited fat accumulation in 3T3-L1 cells, although the effectiveness of [D-Leu(7)]ternatin was lower than that of ternatin. In this study, we investigated the effects of ternatin and [D-Leu(7)]ternatin on obesity and type 2 diabetes in KK-A(y) mice, an animal model for spontaneously developed type 2 diabetes. We continuously administered ternatin (8.5 or 17 nmol/day) or [D-Leu(7)]ternatin (68 nmol/day) to mice via a subcutaneous osmotic pump. Unexpectedly, neither ternatin nor [D-Leu(7)]ternatin affected body weight or adipose tissue weight in KK-A(y) mice. In contrast, it was demonstrated that both ternatin and [D-Leu(7)]ternatin suppress the development of hyperglycemia. In liver, the SREBP-1c mRNA level tended to be lower or significantly decreased in mice treated with ternatin or [D-Leu(7)]ternatin, respectively. Moreover, we found that ternatin directly lowered the SREBP-lc mRNA level in Hepa1-6 hepatocyte cells. This study showed that ternatin and [D-Leu(7)]ternatin each had a preventive effect on hyperglycemia and a suppressive effect on fatty acid synthesis in KK-A(y) mice. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：299 / 304

页数：6

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