Ternatin, a cyclic peptide isolated from mushroom, and its derivative suppress hyperglycemia and hepatic fatty acid synthesis in spontaneously diabetic KK-Ay mice

被引:16
作者
Kobayashi, Misato [1 ]
Kawashima, Haruna [1 ]
Takemori, Kumiko [2 ]
Ito, Hiroyuki [3 ]
Murai, Atsushi [1 ]
Masuda, Shun [4 ]
Yamada, Kaoru [4 ]
Uemura, Daisuke [4 ]
Horio, Fumihiko [1 ]
机构
[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Biosci, Nagoya, Aichi 4648601, Japan
[2] Kinki Univ, Fac Agr, Dept Food & Nutr, Nara 6318505, Japan
[3] Kinki Univ, Dept Biomed Engn, Fac Biol Oriented Engn, Wakayama 6496493, Japan
[4] Kanagawa Univ, Dept Chem, Kanagawa 2591293, Japan
基金
日本科学技术振兴机构;
关键词
Ternatin; Cyclic peptide; Anti-diabetic; Blood glucose; Triglyceride; KK-A(y) mice; INSULIN-RESISTANCE; (-)-TERNATIN; ACCUMULATION; OBESITY; GINSENG; BERRY; PREVENTION; EXTRACT;
D O I
10.1016/j.bbrc.2012.09.045
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
(-)-Ternatin is a highly methylated cyclic heptapeptide isolated from mushroom Coriolus versicolor. Ternatin has an inhibitory effect on fat accumulation in 3T3-L1 adipocytes. [D-Leu(7)]ternatin, a ternatin derivative, also inhibited fat accumulation in 3T3-L1 cells, although the effectiveness of [D-Leu(7)]ternatin was lower than that of ternatin. In this study, we investigated the effects of ternatin and [D-Leu(7)]ternatin on obesity and type 2 diabetes in KK-A(y) mice, an animal model for spontaneously developed type 2 diabetes. We continuously administered ternatin (8.5 or 17 nmol/day) or [D-Leu(7)]ternatin (68 nmol/day) to mice via a subcutaneous osmotic pump. Unexpectedly, neither ternatin nor [D-Leu(7)]ternatin affected body weight or adipose tissue weight in KK-A(y) mice. In contrast, it was demonstrated that both ternatin and [D-Leu(7)]ternatin suppress the development of hyperglycemia. In liver, the SREBP-1c mRNA level tended to be lower or significantly decreased in mice treated with ternatin or [D-Leu(7)]ternatin, respectively. Moreover, we found that ternatin directly lowered the SREBP-lc mRNA level in Hepa1-6 hepatocyte cells. This study showed that ternatin and [D-Leu(7)]ternatin each had a preventive effect on hyperglycemia and a suppressive effect on fatty acid synthesis in KK-A(y) mice. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:299 / 304
页数:6
相关论文
共 21 条
[1]   Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component [J].
Attele, AS ;
Zhou, YP ;
Xie, JT ;
Wu, JA ;
Zhang, L ;
Dey, L ;
Pugh, W ;
Rue, PA ;
Polonsky, KS ;
Yuan, CS .
DIABETES, 2002, 51 (06) :1851-1858
[2]   Selective versus total insulin resistance: A pathogenic paradox [J].
Brown, Michael S. ;
Goldstein, Joseph L. .
CELL METABOLISM, 2008, 7 (02) :95-96
[3]   Soy, phytoestrogens and metabolism: A review [J].
Cederroth, Christopher R. ;
Nef, Serge .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2009, 304 (1-2) :30-42
[4]   Anti-hyperglycemic effects of ginseng: Comparison between root and berry [J].
Dey, L ;
Xie, JT ;
Wang, A ;
Wu, J ;
Maleckar, SA ;
Yuan, CS .
PHYTOMEDICINE, 2003, 10 (6-7) :600-605
[5]  
FOLCH J, 1957, J BIOL CHEM, V226, P497
[6]   Laboratory, Epidemiological, and Human Intervention Studies Show That Tea (Camellia sinensis) May Be Useful in the Prevention of Obesity [J].
Grove, Kimberly A. ;
Lambert, Joshua D. .
JOURNAL OF NUTRITION, 2010, 140 (03) :446-453
[7]   (-)-Ternatin inhibits adipogenesis and lipid metabolism in 3T3-L1 cells [J].
Ito, Masahiko ;
Ito, Junko ;
Kitazawa, Hidefumi ;
Shimamura, Ken ;
Fukami, Takehiro ;
Tokita, Shigeru ;
Shimokawa, Kenichiro ;
Yamada, Kaoru ;
Kanatani, Akio ;
Uemura, Daisuke .
PEPTIDES, 2009, 30 (06) :1074-1081
[8]   Mechanisms linking obesity to insulin resistance and type 2 diabetes [J].
Kahn, Steven E. ;
Hull, Rebecca L. ;
Utzschneider, Kristina M. .
NATURE, 2006, 444 (7121) :840-846
[9]   Anti-obese action of raspberry ketone [J].
Morimoto, C ;
Satoh, Y ;
Hara, M ;
Inoue, S ;
Tsujita, T ;
Okuda, H .
LIFE SCIENCES, 2005, 77 (02) :194-204
[10]   Constituents from the leaves of nelumbo nucifera stimulate lipolysis in the white adipose tissue of mice [J].
Ohkoshi, Emika ;
Miyazaki, Hiromi ;
Shindo, Kazutoshi ;
Watanabe, Hiroyuki ;
Yoshida, Aruto ;
Yajima, Hiroaki .
PLANTA MEDICA, 2007, 73 (12) :1255-1259