Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples

被引：22

作者：

Graca, Didia Coelho ^{[1
]}

Lescuyer, Pierre ^{[1
,2
]}

Clerici, Lorella ^{[2
]}

Tsybin, Yury O. ^{[3
]}

Hartmer, Ralf ^{[4
]}

Meyer, Markus ^{[4
]}

Samii, Kaveh ^{[5
]}

Hochstrasser, Denis F. ^{[1
,2
]}

Scherl, Alexander ^{[1
]}

机构：

[1] Univ Geneva, Fac Med, Dept Human Prot Sci, Biomed Prote Res Grp, Geneva, Switzerland

[2] Univ Hosp Geneva, Dept Genet & Lab Med, Geneva, Switzerland

[3] Ecole Polytech Fed Lausanne, Biomol Mass Spectrometry Lab, Lausanne, Switzerland

[4] Bruker Daltonics Inc, Bremen, Germany

[5] Univ Hosp Geneva, Div Hematol, Geneva, Switzerland

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2012年 / 23卷 / 10期

关键词：

Electron transfer dissociation; Single reaction monitoring; Top-down mass spectrometry; Hemoglobin; Clinical proteomics;

D O I：

10.1007/s13361-012-0446-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whole blood without previous proteolytic cleavage. The analysis was performed in a robust ion trap mass spectrometer operating at nominal mass accuracy and resolution. Subtle differences in globin sequences, resulting with mass shifts of about one Da, can be unambiguously identified. These results suggest that mass spectrometry analysis of entire proteins using electron transfer dissociation can be employed on clinical samples in a workflow compatible with diagnostic applications.

引用

页码：1750 / 1756

页数：7

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