The radiosensitivity of endothelial cells isolated from human breast cancer and normal tissue in vitro

被引：17

作者：

Park, Moon-Taek ^{[1
]}

Oh, Eun-Taex ^{[1
]}

Song, Min-Jeong ^{[1
]}

Kim, Woo-Jean ^{[2
,3
]}

Cho, Young Up ^{[4
]}

Kim, Sei Joong ^{[4
]}

Han, Jee-Young ^{[5
]}

Suh, Jun-Kyu ^{[2
,3
]}

Choi, Eun Kyung ^{[6
]}

Lim, Byung Uk ^{[1
]}

Song, Chang Won ^{[7
]}

Park, Heon Joo ^{[1
]}

机构：

[1] Inha Univ, Coll Med, Ctr Adv Med Educ, Dept Microbiol,Project BK21, Inchon 400712, South Korea

[2] Inha Univ, Coll Med, Dept Urol, Inchon 400712, South Korea

[3] Inha Univ, Coll Med, Natl Res Lab Regenerat Sexual Med, Inchon 400712, South Korea

[4] Inha Univ, Coll Med, Dept Surg, Inchon 400712, South Korea

[5] Inha Univ, Coll Med, Dept Pathol, Inchon 400712, South Korea

[6] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Radiat Oncol, Seoul 138736, South Korea

[7] Univ Minnesota, Dept Therapeut Radiol Radiat Oncol, Minneapolis, MN USA

来源：

MICROVASCULAR RESEARCH | 2012年 / 84卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

TUMOR RESPONSE; GROWTH-FACTOR; ANGIOGENESIS; RADIATION; RECEPTOR; DISEASE; MARKERS; ABNORMALITIES; RADIOTHERAPY; XENOGRAFTS;

D O I：

10.1016/j.mvr.2012.06.002

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

We developed a novel method for harvesting endothelial cells from blood vessels of freshly obtained cancer and adjacent normal tissue of human breast, and compared the response of the cancer-derived endothelial cells (CECs) and normal tissue-derived endothelial cells (NECs) to ionizing radiation. In brief, when tissues were embedded in Matrigel and cultured in endothelial cell culture medium (ECM) containing growth factors, endothelial cells grew out of the tissues. The endothelial cells were harvested and cultured as monolayer cells in plates coated with gelatin, and the cells of 2nd-5th passages were used for experiments. Both CECs and NECs expressed almost the same levels of surface markers CD31, CD105 and TEM-8 (tumor endothelial marker-8), which are known to be expressed in angiogenic endothelial cells, i.e., mitotically active endothelial cells. Furthermore, both CECs and NECs were able to migrate into experimental wound in the monolayer culture, and also to form capillary-like tubes on Matrigel-coated plates. However, the radiation-induced suppressions of migration and capillary-like tube formations were greater for CECs than NECs from the same patients. In addition, in vitro clonogenic survival assays demonstrated that CECs were far more radiosensitive than NECs. In summary, we have developed a simple and efficient new method for isolating endothelial cells from cancer and normal tissue, and demonstrated for the first time that endothelial cells of human breast cancer are significantly more radiosensitive than their normal counterparts from the same patients. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：140 / 148

页数：9