Isolation and characterization of Streptomyces spp. strains F-6 and F-7 capable of decomposing alkali lignin

被引：32
作者：
Yang, Y. S. [1 ,2 ]
Zhou, J. T. [1 ]
Lu, H. [1 ]
Yuan, Y. L. [2 ]
Zhao, L. H. [1 ]
机构：
[1] Dalian Univ Technol, Sch Environm Sci & Technol, Dalian 116024, Peoples R China
[2] Dalian Univ Technol, Sch Environm & Chem Engn, Dalian 116622, Peoples R China
来源：
ENVIRONMENTAL TECHNOLOGY | 2012年 / 33卷 / 23期
关键词：
Streptomyces; alkali lignin; biodegradation; co-culture; GC-MS analysis; SOLID-STATE FERMENTATION; EXTRACELLULAR ENZYMES; KRAFT LIGNIN; DEGRADATION; DECOLORIZATION; BIODEGRADATION; LIGNOCELLULOSE; STRATEGIES; RESIDUES; SOFTWOOD;
D O I：
10.1080/09593330.2012.672473
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Biodegradation and bioconversion of lignin are the result of the combined action of fungi, bacteria and actinomycetes. Through screening from forest soil, two novel isolated actinomycete strains were identified as Streptomyces spp. strains F-6 and F-7 by their morphology, cultural characteristics and high homology to the 16S rRNA gene. Both strains possessed laccase and manganese peroxidase activities. Laccase activity produced by strain F-6 was up to 935.4Ug(-1) dry cell weight. More than 50% of alkali lignin was removed by strains F-6 and F-7 in 12 days of incubation. GC-MS analysis of the biodegraded products showed strain F-6 converted lignin into phenol and broken phenol compounds. The two strains could co-culture with white-rot fungus, and the combined actinonycete-fungus system decomposed alkali lignin effectively.
引用
收藏
页码：2603 / 2609
页数：7
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