Isolation and characterization of Streptomyces spp. strains F-6 and F-7 capable of decomposing alkali lignin

被引：32

作者：

Yang, Y. S. ^{[1
,2
]}

Zhou, J. T. ^{[1
]}

Lu, H. ^{[1
]}

Yuan, Y. L. ^{[2
]}

Zhao, L. H. ^{[1
]}

机构：

[1] Dalian Univ Technol, Sch Environm Sci & Technol, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Environm & Chem Engn, Dalian 116622, Peoples R China

来源：

ENVIRONMENTAL TECHNOLOGY | 2012年 / 33卷 / 23期

关键词：

Streptomyces; alkali lignin; biodegradation; co-culture; GC-MS analysis; SOLID-STATE FERMENTATION; EXTRACELLULAR ENZYMES; KRAFT LIGNIN; DEGRADATION; DECOLORIZATION; BIODEGRADATION; LIGNOCELLULOSE; STRATEGIES; RESIDUES; SOFTWOOD;

D O I：

10.1080/09593330.2012.672473

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Biodegradation and bioconversion of lignin are the result of the combined action of fungi, bacteria and actinomycetes. Through screening from forest soil, two novel isolated actinomycete strains were identified as Streptomyces spp. strains F-6 and F-7 by their morphology, cultural characteristics and high homology to the 16S rRNA gene. Both strains possessed laccase and manganese peroxidase activities. Laccase activity produced by strain F-6 was up to 935.4Ug(-1) dry cell weight. More than 50% of alkali lignin was removed by strains F-6 and F-7 in 12 days of incubation. GC-MS analysis of the biodegraded products showed strain F-6 converted lignin into phenol and broken phenol compounds. The two strains could co-culture with white-rot fungus, and the combined actinonycete-fungus system decomposed alkali lignin effectively.

引用

页码：2603 / 2609

页数：7

共 33 条

[1] PRODUCTION OF MAJOR EXTRACELLULAR ENZYMES DURING LIGNOCELLULOSE DEGRADATION BY 2 STREPTOMYCETES IN AGITATED SUBMERGED CULTURE [J].

ADHI, TP ;

KORUS, RA ;

CRAWFORD, DL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (05) :1165-1168

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3] DEGRADATION OF SOFTWOOD, HARDWOOD, AND GRASS LIGNOCELLULOSES BY 2 STREPTOMYCES STRAINS [J].

ANTAI, SP ;

CRAWFORD, DL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1981, 42 (02) :378-380

[4]

Antonopoulos VT, 2001, APPL MICROBIOL BIOT, V57, P92

[5] Solid-state fermentation of wood residues by Streptomyces griseus B1, a soil isolate, and solubilization of lignins [J].

Arora, A ;

Nain, L ;

Gupta, J .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2005, 21 (03) :303-308

[6] Optimization of medium for decolorization of Solar golden yellow R direct textile dye by Schizophyllum commune IBL-06 [J].

Asgher, Muhammad ;

Kausar, Shahnaz ;

Bhatti, Haq Nawaz ;

Shah, Syed Agha Hassan ;

Ali, Muhammad .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2008, 61 (02) :189-193

[7] Lignin peroxidase isoforms from Streptomyces viridosporus T7A:: are they a monomer based structure? [J].

Bon, EPS ;

Nascimento, HJ ;

Macedo, JMB ;

Silva, JG .

BIOTECHNOLOGY TECHNIQUES, 1999, 13 (05) :289-293

[8]

CPPA, 1974, H5P CPPA

[9] PRODUCTION OF AN AROMATIC ALDEHYDE OXIDASE BY STREPTOMYCES-VIRIDOSPORUS [J].

CRAWFORD, DL ;

SUTHERLAND, JB ;

POMETTO, AL ;

MILLER, JM .

ARCHIVES OF MICROBIOLOGY, 1982, 131 (04) :351-355

[10] Purification and characterization of two extracellular peroxidases from Streptomyces sp strain AM2, a decolorizing actinomycetes responsible for the biodegradation of natural humic acids [J].

Fodil, Djamila ;

Badis, Abdelmalek ;

Jaouadi, Bassem ;

Zarai, Nedia ;

Ferradji, Fatma Zohra ;

Boutoumi, Houcine .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2011, 65 (03) :470-478

← 1 2 3 4 →