Proteolytic Activity at Quantum Dot-Conjugates: Kinetic Analysis Reveals Enhanced Enzyme Activity and Localized Interfacial "Hopping"

被引:115
作者
Algar, W. Russ [1 ,3 ]
Malonoski, Anthony [1 ]
Deschamps, Jeffrey R. [1 ]
Banco-Canosa, Juan B. [4 ,5 ]
Susumu, Kimihiro [2 ]
Stewart, Michael H. [2 ]
Johnson, Brandy J. [1 ]
Dawson, Philip E. [4 ,5 ]
Medintz, Igor L. [1 ]
机构
[1] USN, Ctr Bio Mol Sci & Engn, Res Lab, Washington, DC 20375 USA
[2] USN, Div Opt Sci, Res Lab, Washington, DC 20375 USA
[3] George Mason Univ, Coll Sci, Fairfax, VA 22030 USA
[4] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
[5] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
基金
加拿大自然科学与工程研究理事会;
关键词
Quantum dot; nanoparticle; peptide; proteolysis; kinetics; FRET; RESONANCE ENERGY-TRANSFER; BIOCOMPATIBLE SEMICONDUCTOR; QUANTITATIVE-ANALYSIS; GOLD NANOPARTICLES; STABILITY; LIGANDS; CHYMOTRYPSIN; PARAMETERS; INHIBITORS; CATALYSIS;
D O I
10.1021/nl301727k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Recent studies show that polyvalent, ligand-modified nanoparticles provide significantly enhanced binding characteristics compared to isolated ligands. Here, we assess the ability of substrate-modified nanoparticles to provide enhanced enzymatic activity. Energy transfer assays allowed quantitative, real-time measurement of proteolytic digestion at polyvalent quantum dot-peptide conjugates. Enzymatic progress curves were analyzed using an integrated Michaelis Menten (MM) formalism, revealing mechanistic details, including deviations from classic MM-behavior. A "hopping" mode of proteolysis at the nanoparticle was identified, confirming enhanced activity.
引用
收藏
页码:3793 / 3802
页数:10
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