Lignin peroxidase isoforms from Streptomyces viridosporus T7A:: are they a monomer based structure?

被引：5

作者：

Bon, EPS ^{[1
]}

Nascimento, HJ ^{[1
]}

Macedo, JMB ^{[1
]}

Silva, JG ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Dept Bioquim, Inst Quim, CT,Ilha Fdn, BR-21949900 Rio De Janeiro, Brazil

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 05期

关键词：

isoforms characterization; lignin peroxidase; molecular weight; polymeric structure; Streptomyces viridosporus;

D O I：

10.1023/A:1006914312208

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five fractions with lignin peroxidase activity were isolated by FPLC-Mono Q from a Streptomyces viridosporus culture. F4 and F5 showed the highest specific activity and degree of protein homogeneity by chromatofocusing, IEF- and gradient-PAGE. The individual analysis of F4 and F5 by FPLC-Superdex 75, showed MW that were multiples to each other (68,000; 23,000; 12,000), although by SDS PAGE a sole MW of 13,500 was obtained, indicating a monomer based structure. The amino-acid composition of F5 showed absence of sulfur amino acids.

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页码：289 / 293

页数：5

相关论文

共 13 条

[1] PRODUCTION OF MAJOR EXTRACELLULAR ENZYMES DURING LIGNOCELLULOSE DEGRADATION BY 2 STREPTOMYCETES IN AGITATED SUBMERGED CULTURE [J].

ADHI, TP ;

KORUS, RA ;

CRAWFORD, DL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (05) :1165-1168

[2] PRODUCTION AND SECRETION OF PROTEINS BY STREPTOMYCETES [J].

GIBERT, M ;

MOROSOLI, R ;

SHARECK, F ;

KLUEPFEL, D .

CRITICAL REVIEWS IN BIOTECHNOLOGY, 1995, 15 (01) :13-39

[3] IMMUNOLOGICAL RELATEDNESS OF EXTRACELLULAR LIGNINASES FROM THE ACTINOMYCETES STREPTOMYCES-VIRIDOSPORUS T7A AND STREPTOMYCES-BADIUS 252 [J].

MAGNUSON, TS ;

ROBERTS, MA ;

CRAWFORD, DL ;

HERTEL, G .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1991, 28-9 :433-443

[4] PURIFICATION AND CHARACTERIZATION OF AN INTRACELLULAR PEROXIDASE FROM STREPTOMYCES-CYANEUS [J].

MLIKI, A ;

ZIMMERMANN, W .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (03) :916-919

[5]

Odier E., 1992, Microbial degradation of natural products., P161

[6] THE EFFECTS OF VARIOUS NUTRIENTS ON EXTRACELLULAR PEROXIDASES AND ACID-PRECIPITABLE POLYMERIC LIGNIN PRODUCTION BY STREPTOMYCES-CHROMOFUSCUS A2 AND S-VIRIDOSPORUS T7A [J].

PASTI, MB ;

HAGEN, SR ;

KORUS, RA ;

CRAWFORD, DL .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1991, 34 (05) :661-667

[7] SIMPLIFICATION OF PROTEIN ASSAY METHOD OF LOWRY ET AL - WHICH IS MORE GENERALLY APPLICABLE [J].

PETERSON, GL .

ANALYTICAL BIOCHEMISTRY, 1977, 83 (02) :346-356

[8] EXTRACELLULAR ENZYME-ACTIVITIES DURING LIGNOCELLULOSE DEGRADATION BY STREPTOMYCES SPP - A COMPARATIVE-STUDY OF WILD-TYPE AND GENETICALLY MANIPULATED STRAINS [J].

RAMACHANDRA, M ;

CRAWFORD, DL ;

POMETTO, AL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (12) :2754-2760

[9] CHARACTERIZATION OF AN EXTRACELLULAR LIGNIN PEROXIDASE OF THE LIGNOCELLULOLYTIC ACTINOMYCETE STREPTOMYCES-VIRIDOSPORUS [J].

RAMACHANDRA, M ;

CRAWFORD, DL ;

HERTEL, G .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (12) :3057-3063

[10] Production and partial characterization of extracellular peroxidases produced by Streptomyces avermitilis UAH30 [J].

Rob, A ;

Hernandez, M ;

Ball, AS ;

Tuncer, M ;

Arias, ME ;

Wilson, MT .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 62 (2-3) :159-174