New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics

被引:18
作者
Zhang, Yu [1 ,2 ,3 ,4 ,5 ]
Zhao, Peng [6 ]
Yue, Shidong [1 ,2 ,3 ,4 ,5 ]
Liu, Mingjie [1 ,2 ,3 ,4 ,5 ]
Qiao, Yongliang [1 ,2 ,3 ,4 ,7 ]
Xu, Shaochun [1 ,2 ,3 ,4 ,5 ]
Gu, Ruiting [1 ,2 ,3 ,4 ,5 ]
Zhang, Xiaomei [1 ,2 ,3 ,4 ,5 ]
Zhou, Yi [1 ,2 ,3 ,4 ,5 ]
机构
[1] Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
[3] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
[4] Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China
[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[6] Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
[7] Qingdao Univ Sci & Technol, Qingdao 266000, Peoples R China
基金
中国国家自然科学基金;
关键词
Anoxia; Physiological response; Submerged aquatic vegetation; Seagrass; Photosynthesis and growth; Transciiptomics; Metabolomics; Zostera marina L; Eelgrass; LOW-OXYGEN RESPONSE; RICE; EELGRASS; ADAPTATIONS; METABOLISM; MECHANISMS; SEEDLINGS; GROWTH; ARABIDOPSIS; ECHINOCHLOA;
D O I
10.1016/j.scitotenv.2020.144717
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Coastal hypoxia/anoxia is a major emerging threat to global coastal ecosystems. Macroalgae blooms of tens of kilometers are often observed in open waters. These blooms not only cause a lack of oxygen, but also benthic light limitation. We explored the physiological responses of Zostera marina L to anoxia under darkness. After exposing Z. marina to anoxia under darkness for 72 h, we measured the elongation of leaves and the decrease in maximal quantum yield of photosystem II (Fv/Fm), and investigated the transcriptomic and metabolomic responses to anoxic stress based on RNA-sequencing and liquid chromatography-mass spectrometry (LC-MS) technology. The results showed that anoxic stress significantly reduced the leaf Fv/Fm, and had a significant negative effect on the photosynthesis and growth of Z. marina. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of up-regulated differentially expressed genes (DEGs) showed that glycolysis was the most significant enrichment pathway (p < 0.001), and most of the important products in glycolysis were significantly up-regulated. This indicated that the glycolysis process of anaerobic respiration is promoted under anoxia The metabolite results also showed that glyceraldehyde 3-phosphate in the glycolysis pathway was significantly up-regulated. Moreover, three genes encoding sucrose synthase (gene-ZOSMA_310G00150, gene-ZOSMA_81G00980, and gene-ZOSMA_8G00730) and one gene encoding alpha-amylase (gene-ZOSMA_95G00270) were significantly up-regulated, providing the sugar basis for the subsequent increase in glycolysis. Furthermore, gene-encoding oxoglutarate dehydrogenase, the rate-limiting step of the tricarboxylic add (TCA) cycle, was significantly down-regulated, indicating that this cycle was inhibited under anoxia. Metabolomic results showed that L-tryptophan, L-phenylalanine, and DL-leucine were significantly upregulated. Only significantly decreased glutamate and non-significantly decreased glutamine, substances consumed in alanine and gamma-aminobutyric acid (GABA) shunt mechanisms, were detected in the leaves, while GABA and alanine were not detected. The results of this study show that anoxic stress induces a programmed transcriptomic and metabolomic response in seagrass, most likely reflecting a complex strategy of acclimation and adaptation in seagrass to resist anoxic stress. (C) 2021 Elsevier B.V. All rights reserved.
引用
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页数:11
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