Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury

被引：14

作者：

Xie, Haibo ^{[1
,2
]}

Wang, Hui ^{[1
,2
]}

Wu, Qifeng ^{[3
]}

Peng, Jiale ^{[4
]}

Huang, Hua ^{[4
]}

Wang, Yican ^{[4
]}

Huang, Meng ^{[4
]}

Jiang, Wei ^{[4
]}

Yang, Yi ^{[4
]}

Zhang, Xuesong ^{[4
]}

Zhang, Jiaxiang ^{[2
,4
]}

Zhu, Qixing ^{[1
,2
]}

机构：

[1] Anhui Med Univ, Dept Dermatol, Affiliated Hosp 1, Hefei, Anhui, Peoples R China

[2] Anhui Med Univ, Minist Educ, Key Lab Dermatol, Hefei, Peoples R China

[3] Guangdong Prov Hosp Occupat Dis Prevent & Treatme, Guangzhou, Guangdong, Peoples R China

[4] Anhui Med Univ, Sch Publ Hlth, Dept Occupat Hlth & Environm Hlth, Hefei, Anhui, Peoples R China

来源：

JOURNAL OF INFLAMMATION RESEARCH | 2021年 / 14卷

基金：

中国国家自然科学基金;

关键词：

trichloroethylene; glomerular; endothelin-1; endothelin receptor type A; angiopoietin-1; angiopoietin-2; ANGIOPOIETIN-1; TCE;

D O I：

10.2147/JIR.S301104

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Introduction: This study aimed to investigate the mechanism in regulating the cross talk between glomerular endothelial cells and podocytes in "occupational medicamentosa-like dermatitis induced by trichloroethylene (OMLDT)" patients. Methods: Totally 6 OMLDT patients, 18 controls, and 102 BALB/c female mice were involved in this study. Patient's serum endothelin-1 (ET-1), angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), blood urea nitrogen (BUN), and podocalyxin (PCX) were detected. All the mice were used to establish the trichloroethylene (TCE) sensitized mouse model. Transmission electron microscope results were used to reflect renal glomerulus injury. Protein levels were detected by Western blot. Ang-1/Ang-2 gene level was reflected by RTPCR. Cell apoptosis level was detected by using TUNEL assay kit. Results: We found that in OMLDT patients, ET-1, Ang-2, BUN, and PCX were highly expressed but Ang-1 was inhibited. In TCE sensitized positive mouse, the downregulation of Ang-1, pTie-2 and the upregulation of Ang-2 were mediated by ET-1/ETAR but not ET-1/ETBR. The promotor of apoptosis proteins was downregulated and the inhibitor of apoptosis proteins was upregulated by treating with BQ123. Discussion: ET-1/ETAR-Angs/Tie-2 pathway mediated the cross talk between glomerular endothelial cells and podocytes. BQ123 can alleviate glomerulus immune injury.

引用

页码：761 / 776

页数：16