Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism

被引：5

作者：

Huang, Gui-Li ^{[1
]}

Sun, Ling-Xiang ^{[1
]}

Ma, Jia-Jia ^{[1
]}

Sui, Si-Yao ^{[1
]}

Wang, Yu-Ning ^{[1
,2
]}

机构：

[1] Suzhou Acad Agr Sci, Agr Prod Storage & Proc Lab, Suzhou, Peoples R China

[2] Inst Chem Ind Forest Prod CAF, Jiangsu Key Lab Biomass Energy & Mat, Nanjing, Peoples R China

来源：

BIOENGINEERED | 2021年 / 12卷 / 01期

关键词：

Polyphenol oxidase; total flavonoids; young loquat fruits; inhibitory mechanism; BOVINE SERUM-ALBUMIN; TYROSINASE INHIBITORS; PROANTHOCYANIDINS; ANTIOXIDANT; INSIGHTS; BINDING; POTATO;

D O I：

10.1080/21655979.2021.1886387

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC (50) value of 21.03 +/- 2.37 mu g/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry.

引用

页码：640 / 647

页数：8

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