DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus

被引：23
|
作者：
Antwerpen, Markus H.
Schellhase, Michaela
Ehrentreich-Foerster, Eva
Bier, Frank
Witte, Wolfgang
Nuebel, Ulrich
机构：
[1] Robert Koch Inst, D-38855 Wernigerode, Germany
[2] Fraunhofer Inst Biomed Tech, Nuthetal, Germany
来源：
MOLECULAR AND CELLULAR PROBES | 2007年 / 21卷 / 02期
关键词：
diagnostic microarray; Bacillus anthracis; Bacillus cereus; ATCC; 10987; antibiotic resistance; ciprofloxacin; doxycycline; rifampin; vancomycin;
D O I：
10.1016/j.mcp.2006.10.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a multiplex PCR for amplification of ten genes involved in resistance to ciprofloxacin, doxycycline, rifampin, and vancomycin in Bacillus anthracis and closely related Bacillus cereus. Enzymatic labelling of PCR products followed by hybridization to oligonucleotide probes on a DNA microarray enabled simultaneous detection of resistance genes tetK, tetL, tetM, tetO, vanA, and vanB and resistance-mediating point mutations in genes gyrA, gyrB, parC, and rpoB. The presented assay allows detection of clinically relevant antibiotic resistance determinants within 4 h and can be used as a time-saving tool supporting conventional culture-based diagnostics. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：152 / 160
页数：9
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