Clonorchis sinensis:: Development and evaluation of a nested polymerase chain reaction (PCR) assay

被引：38

作者：

Parvathi, Ammini

Kumar, H. Sanath

Prakasha, B. Kencharma

Lu, Jieyuan

Xu, Xueman

Hu, Wei

Feng, Zheng

Karunasagar, Indrani ^{[1
]}

Karunasagar, Iddya

机构：

[1] Karnataka Vet Anim & Fisheries Sci Univ, Dept Fishery Microbiol, Coll Fisheries, Mangalore 575002, India

[2] Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China

来源：

EXPERIMENTAL PARASITOLOGY | 2007年 / 115卷 / 03期

关键词：

Clonorchis sinensis; trematode; PCR; seafood;

D O I：

10.1016/j.exppara.2006.09.010

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C sinensis. The primers designed from the nucleotide sequence data derived in this study were evaluated for their specificity and sensitivity for the detection of C sinensis. The specific amplification products were obtained only with C sinensis and no amplifications occurred with the DNA of closely related trematodes including Opisthorchis viverrini demonstrating the specificity of the assay. The novel PCR method described here will be useful for the quarantine of fishery products and evaluation of transmission status of clonorchiasis in the endemic areas. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：291 / 295

页数：5

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