The Study on Biocompatibility of Porous nHA/PLGA Composite Scaffolds for Tissue Engineering with Rabbit Chondrocytes In Vitro

被引：13

作者：

Chen, Lei ^{[1
,2
,3
]}

Zhu, Wei-Min ^{[2
,3
]}

Fei, Zhi-Qiang ^{[2
,3
]}

Chen, Jie-Lin ^{[2
,3
]}

Xiong, Jian-Yi ^{[2
,3
]}

Zhang, Ju-Feng ^{[2
,3
]}

Duan, Li ^{[2
,3
]}

Huang, Jianghong ^{[2
,3
]}

Liu, Zhiyong ^{[2
,3
]}

Wang, Daping ^{[1
,2
,3
]}

Zeng, Yanjun ^{[4
]}

机构：

[1] Guangzhou Med Univ, Guangzhou 510182, Guangdong, Peoples R China

[2] Second Peoples Hosp Shenzhen, Dept Orthoped, Shenzhen 518035, Guangdong, Peoples R China

[3] Shenzhen Key Lab Tissue Engn, Shenzhen 518035, Guangdong, Peoples R China

[4] Beijing Univ Technol, Biomech & Med Informat Inst, Beijing 100022, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2013年 / 2013卷

关键词：

BONE; CELLS;

D O I：

10.1155/2013/412745

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objective. To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering. Methods. Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry. Results. The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05). Conclusion. The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

引用

页数：6

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