Expression and localization of hypoxia-inducible factor-1 subunits in the adult rat epididymis

被引:17
|
作者
Palladino, MA
Powell, JD
Korah, N
Hermo, L
机构
[1] Monmouth Univ, Dept Biol, W Long Branch, Long Branch, NJ 07764 USA
[2] McGill Univ, Dept Anat & Cell Biol, Montreal, PQ H3A 2B2, Canada
关键词
epididymis; gene regulation; male reproductive tract; sperm; stress;
D O I
10.1095/biolreprod.103.023085
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The epididymal epithelium contributes to formation of a luminal fluid that is essential for the protection of spermatozoa from a variety of insults including changes in oxygen tension. A key regulator of the response to oxygen debt in many cells is hypoxia-inducible factor-1 (HIF-1). A transcription factor composed of alpha and beta subunits, HIF-1 activates genes that mediate oxygen homeostasis and cell survival pathways or trigger cell death responses. Previously we have shown that HIF-1alpha mRNA is expressed in the adult rat epididymis. Goals of this study were to determine whether HIF-1alpha protein is activated by ischemia in the rat epididymis, to determine whether epididymal HIF-1alpha mRNA expression is androgen dependent, and to identify epididymal cell types expressing HIF-1alpha and beta. Immunoblot analysis revealed that HIF-1alpha protein is primarily present in corpus and cauda of the normoxic epididymis and unaffected by ischemia, whereas HIF-1beta was detected equally in all regions and also unaffected by ischemia. HIF-1alpha mRNA expression in all regions was not affected by 15 days bilateral orchiectomy. Principal cells stained positive for HIF-1alpha by immunocytochemistry, with the epithelium of initial segment and caput epididymidis staining less intensely than corpus and cauda. HIF-1beta immunoreactivity was equally present in principal cells in all regions. Clear, narrow, and basal cells were unreactive for HIF-1alpha and beta. The presence of HIF-1 in normoxic epididymis and the regional distribution of HIF-1alpha suggests fundamental differences in how proximal and distal regions of the epididymis maintain oxygen homeostasis to protect the epithelium and spermatozoa from hypoxia.
引用
收藏
页码:1121 / 1130
页数:10
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