Coexpression of leptin receptor and preproneuropeptide Y mRNA in arcuate nucleus of mouse hypothalamus

被引：425

作者：

Mercer, JG ^{[1
]}

Hoggard, N ^{[1
]}

Williams, LM ^{[1
]}

Lawrence, CB ^{[1
]}

Hannah, LT ^{[1
]}

Morgan, PJ ^{[1
]}

Trayhurn, P ^{[1
]}

机构：

[1] ROWETT RES INST,MOL PHYSIOL GRP,BUCKSBURN AB2 9SB,ABERDEEN,SCOTLAND

来源：

JOURNAL OF NEUROENDOCRINOLOGY | 1996年 / 8卷 / 10期

关键词：

leptin receptor mRNA; neuropeptide Y mRNA; arcuate nucleus;

D O I：

10.1046/j.1365-2826.1996.05161.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Leptin, the protein product of the adipose tissue-specific ob (obese) gene (1), reduces the body weight, adiposity and food intake of obese ob/ob mice on peripheral or central injection (2, 3, 4), [I-125]leptin binding has been detected in mouse choroid plexus (5), from which a leptin receptor gene was expression cloned (5). The gene has at least 6 splice variants (6, 7), Leptin receptor mRNA was localized in the hypothalamus by in situ hybridization being particularly abundantly expressed in the arcuate nucleus (8), There is evidence linking the physiological effects of injected leptin with hypothalamic neuropeptide Y (9, 10) (NPY), which has potent central effects on food intake and energy balance (11), and is also expressed in the arcuate nucleus. Here we report dual in situ hybridization studies for leptin receptor and NPY gene expression in the mouse arcuate nucleus, where the majority of cells examined expressed both genes. This provides the first direct evidence that leptin acts on cells that express NPY mRNA.

引用

页码：733 / 735

页数：3

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