A tomato ER-type Ca2+-ATPase, LCA1, has a low thapsigargin-sensitivity and can transport manganese

被引：18

作者：

Johnson, Neil A. ^{[1
,2
]}

Liu, Fengli ^{[1
,2
]}

Weeks, Phillip D. ^{[1
,2
]}

Hentzen, Audrey E. ^{[3
]}

Kruse, Hilary P. ^{[1
,2
]}

Parker, Jennifer J. ^{[1
,2
]}

Laursen, Mette ^{[4
]}

Nissen, Poul ^{[4
]}

Costa, Charles J. ^{[1
,2
]}

Gatto, Craig ^{[1
,2
]}

机构：

[1] Illinois State Univ, Sch Biol Sci, Div Biomed Sci & Cell Biol, Normal, IL 61790 USA

[2] Illinois State Univ, Sch Biol Sci, Physiol Sect, Normal, IL 61790 USA

[3] Illinois State Univ, Dept Hlth Sci, Normal, IL 61790 USA

[4] Univ Aarhus, Danish Natl Res Fdn, Ctr Membrane Pumps Cells & Dis PUMPKIN, Dept Mol Biol, DK-8000 Aarhus C, Denmark

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2009年 / 481卷 / 02期

关键词：

P-type ATPase; Ca-pump; Thapsigargin; Membrane transport; Tomato; Yeast; Mn2+ transport; Homology modeling; ENDOPLASMIC RETICULUM-TYPE; YEAST SECRETORY PATHWAY; SARCOPLASMIC-RETICULUM; CALCIUM-PUMP; ATP-BINDING; HETEROLOGOUS EXPRESSION; SIGNAL-TRANSDUCTION; CYCLOPIAZONIC ACID; H+/CA2+ EXCHANGE; CA2+ PUMPS;

D O I：

10.1016/j.abb.2008.11.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant Ca2+-ATPase from tomato (i.e. LCA1 for Lycopersicon esculentum [Since the identification and naming of LCA1, the scientific name for the tomato has been changed to Solarium lycopersicum.) Ca-ATPase) was heterologously expressed in yeast for structure-function characterization. We investigate the differences between plant and animal Ca pumps utilizing comparisons between chicken and rabbit SERCA-type pumps with Arabidopsis (ECA1) and tomato plant (LCA1) Ca2+-ATPases. Enzyme function was confirmed by the ability of each Ca2+-ATPase to rescue K616 growth on EGTA-containing agar and directly via in vitro ATP hydrolysis. We found LCA1 to be similar to 300-fold less sensitive to thapsigargin than animal SERCAs, whereas ECA1 was thapsigargin-resistant. LCA1 showed typical pharmacological sensitivities to cyclopiazonic acid, vanadate, and eosin, consistent with it being a P-IIA-type Ca2+-ATPase. Possible amino acid changes responsible for the reduced plant thapsigargin-sensitivity are discussed. We found that LCA1 also complemented K616 yeast growth in the presence of Mn2+, consistent with moving Mn2+ into the secretory pathway and functionally compensating for the lack of secretory pathway Ca-ATPases (SPCAs) in plants. Published by Elsevier Inc

引用

页码：157 / 168

页数：12