Nonenzymatic Template-Directed RNA Synthesis Inside Model Protocells

被引：238

作者：

Adamala, Katarzyna ^{[1
,2
,3
]}

Szostak, Jack W. ^{[1
,2
]}

机构：

[1] Massachusetts Gen Hosp, Howard Hughes Med Inst, Dept Mol Biol, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA

[3] Univ Roma Tre, Dipartimento Biol, Rome, Italy

来源：

SCIENCE | 2013年 / 342卷 / 6162期

关键词：

CITRATE;

D O I：

10.1126/science.1241888

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Efforts to recreate a prebiotically plausible protocell, in which RNA replication occurs within a fatty acid vesicle, have been stalled by the destabilizing effect of Mg2+ on fatty acid membranes. Here we report that the presence of citrate protects fatty acid membranes from the disruptive effects of high Mg2+ ion concentrations while allowing RNA copying to proceed, while also protecting single-stranded RNA from Mg2+-catalyzed degradation. This combination of properties has allowed us to demonstrate the chemical copying of RNA templates inside fatty acid vesicles, which in turn allows for an increase in copying efficiency by bathing the vesicles in a continuously refreshed solution of activated nucleotides.

引用

页码：1098 / 1100

页数：3

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