Nucleolin is a histone chaperone with FACT-like activity and assists remodeling of nucleosomes

被引：198
作者：
Angelov, Dimitar
Bondarenko, Vladimir A.
Almagro, Sebastien
Menoni, Herve
Mongelard, Fabien
Hans, Fabienne
Mietton, Flore
Studitsky, Vasily M.
Hamiche, Ali
Dimitrov, Stefan [1 ]
Bouvet, Philippe
机构：
[1] INSERM, Inst Albert Bonniot, U309, La Tronche, France
[2] CNRS, Inst Andre Lwoff, UPR 9079, Villejuif, France
[3] Ecole Normale Super Lyon, CNRS, UMR 5161, INRA 1237,IFR Biosci 128, F-69364 Lyon, France
[4] Lab Joliot Curie, Lyon, France
[5] Univ Med & Dent New Jersey, Dept Pharmacol, Piscataway, NJ 08854 USA
来源：
EMBO JOURNAL | 2006年 / 25卷 / 08期
关键词：
macroH2A and H2ABbd histone variants; nucleolin; nucleosome; remodeling; transcription;
D O I：
10.1038/sj.emboj.7601046
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Remodeling machines play an essential role in the control of gene expression, but how their activity is regulated is not known. Here we report that the nuclear protein nucleolin possesses a histone chaperone activity and that this factor greatly enhances the activity of the chromatin remodeling machineries SWI/SNF and ACF. Interestingly, nucleolin is able to induce the remodeling by SWI/SNF of macroH2A, but not of H2ABbd nucleosomes, which are otherwise resistant to remodeling. This new histone chaperone promotes the destabilization of the histone octamer, helping the dissociation of a H2A-H2B dimer, and stimulates the SWI/SNF- mediated transfer of H2A-H2B dimers. Furthermore, nucleolin facilitates transcription through the nucleosome, which is reminiscent of the activity of the FACT complex. This work defines new functions for histone chaperones in chromatin remodeling and regulation of transcription and explains how nucleolin could act on transcription.
引用
收藏
页码：1669 / 1679
页数：11
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