Effect of Oral Glutamine on Enterocyte Turnover during Methotrexate-Induced Mucositis in Rats

被引：19
|
作者：
Sukhotnik, Igor [1 ,2 ]
Mogilner, Jorge G. [2 ]
Karry, Rahel [1 ]
Shamian, Benhoor [1 ]
Lurie, Michael [3 ]
Kokhanovsky, Natalie [2 ]
Ure, Benno M. [4 ]
Coran, Arnold G. [5 ,6 ]
机构：
[1] Bnai Zion Med Ctr, Rappaport Fac Med, Lab Intestinal Adaptat & Recovery, IL-31048 Haifa, Israel
[2] Bnai Zion Med Ctr, Dept Pediat Surg, IL-31048 Haifa, Israel
[3] Bnai Zion Med Ctr, Dept Pathol, IL-31048 Haifa, Israel
[4] Hannover Med Sch, Dept Pediat Surg, D-3000 Hannover, Germany
[5] Univ Michigan, CS Mott Childrens Hosp, Pediat Surg Sect, Ann Arbor, MI 48109 USA
[6] Univ Michigan, Sch Med, Ann Arbor, MI USA
来源：
DIGESTION | 2009年 / 79卷 / 01期
关键词：
Methotrexate; Intestine; Glutamine; Enterocyte; proliferation; Enterocyte apoptosis; SMALL-INTESTINE; INDUCED ENTEROCOLITIS; MUCOSAL INJURY; APOPTOSIS; CELL; REPERFUSION; METABOLISM; ISCHEMIA; GUT;
D O I：
10.1159/000191209
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Background/Aims: The objective of this study was to evaluate the effects of oral glutamine in preventing intestinal mucosal damage caused by methotrexate (MTX) in rats. Methods: Male Sprague-Dawley rats were divided into 3 experimental groups: control rats, rats treated intraperitoneally with MTX (MTX rats) and rats treated with oral glutamine in the drinking water (2%) 72 h following intraperitoneal injection of a single dose of MTX (MTX-glutamine rats). Intestinal mucosal damage (Park's injury score), mucosal structural changes, enterocyte proliferation and enterocyte apoptosis were determined 72 h following MTX injection. RT-PCR was used to determine Bax and Bcl-2 mRNA expression. Results: MTX-glutamine rats demonstrated greater jejunal and ileal mucosal weight and mucosal DNA, greater ileal villus height and crypt depth, and a greater index of proliferation in the jejunum and ileum compared to MTX animals. A significant decrease in enterocyte apoptosis in the ileum of MTX-glutamine rats (vs. MTX) was accompanied by decreased Bax and increased Bcl-2 mRNA expression. Conclusions: Treatment with oral glutamine prevents mucosal injury and improves intestinal recovery following MTX injury in the rat. Copyright (c) 2009 S. Karger AG, Basel
引用
收藏
页码：5 / 13
页数：9
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