Effects of allelochemical extracted from water lettuce (Pistia stratiotes Linn.) on the growth, microcystin production and release of Microcystis aeruginosa

被引:41
作者
Wu, Xiang [1 ]
Wu, Hao [2 ]
Chen, Junren [1 ]
Ye, Jinyun [1 ]
机构
[1] Huzhou Teachers Coll, Coll Life Sci, Huzhou City 313000, Zhejiang, Peoples R China
[2] Environm Protect Monitoring Ctr Stn, Huzhou City 313000, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Pistia stratiotes Linn; Allelochemical; Microcystis aeruginosa; Microcystin-LR; Growth inhibition; Ecological safety; BLUE-GREEN-ALGAE; BARLEY-STRAW; EICHHORNIA-CRASSIPES; TOXIN PRODUCTION; INHIBITION; CYANOBACTERIA; IDENTIFICATION; MACROPHYTES; PHYTOPLANKTON; ZOOPLANKTON;
D O I
10.1007/s11356-013-1783-x
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This study explored the optimisation of a method of extracting allelochemicals from Pistia stratiotes Linn., identified the optimal dose range for the allelochemicals' anti-algal effect and investigated their impact on the growth of Microcystis aeruginosa, as well as the production and release of microcystin-LR (MC-LR). Based on measured changes in algal cell density and chlorophyll a (Chl-a) content, the allelochemicals were confirmed to have the strongest anti-algal effect with the lowest half-effect concentration of 65 mg L-1 when they were extracted using ethyl acetate as the extraction solvent, 1:20 g mL(-1) as the extraction ratio and 1 h as the extraction time. The allelochemicals extracted from P. stratiotes using this optimal method exhibited the strongest inhibitory effect on the growth of algae when used within a dose range of 60-100 mg L-1; the relative inhibitory ratio reached 50-90 %, and Chl-a content reduced 50-75 % in algae cell cultures within 3-7 days. In addition, the extracted allelochemical compounds demonstrated no significant impact on the extracellular release of MC-LR during the culturing period. The amount of intracellular MC-LR per 10(6) algal cells increased depending on the increasing dose of allelochemicals from P. stratiotes after 7 days of culturing and maintained stability after 16 days. There was no increase in the total amount of MC-LR in the algal cell culture medium. Therefore, the application of allelochemicals from P. stratiotes to inhibit M. aeruginosa has a high degree of ecological safety and can be adopted in practical applications for treating water subjected to algae blooms because the treatment can effectively inhibit the proliferation of algal cells without increasing the release of cyanotoxin.
引用
收藏
页码:8192 / 8201
页数:10
相关论文
共 52 条
[41]   Impact of submerged macrophytes including charophytes on phyto- and zooplankton communities: allelopathy versus other mechanisms [J].
van Donk, E ;
van de Bund, WJ .
AQUATIC BOTANY, 2002, 72 (3-4) :261-274
[42]   Allelochemicals as leads for new herbicides and agrochemicals [J].
Vyvyan, JR .
TETRAHEDRON, 2002, 58 (09) :1631-1646
[43]   Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes [J].
Wang, Jing ;
Zhu, Junying ;
Liu, Shaoping ;
Liu, Biyun ;
Gao, Yunni ;
Wu, Zhenbin .
CHEMOSPHERE, 2011, 85 (06) :977-982
[44]   Effects of light on the microcystin content of Microcystis strain PCC 7806 [J].
Wiedner, C ;
Visser, PM ;
Fastner, J ;
Metcalf, JS ;
Codd, GA ;
Mur, LR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) :1475-1481
[45]  
[吴程 WU Cheng], 2008, [生态学报, Acta Ecologica Sinica], V28, P2595
[46]  
Wu X, 2010, J SOIL WATER CONSERV, V24, P51
[47]   Isolation and identification of antialgal compounds from the leaves of Vallisneria spiralis L. by activity-guided fractionation [J].
Xian, Qiming ;
Chen, Haidong ;
Liu, Huili ;
Zou, Huixian ;
Yin, Daqiang .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2006, 13 (04) :233-237
[48]   Effects of Tibetan hulless barley on bloom-forming cyanobacterium (Microcystis aeruginosa) measured by different physiological and morphologic parameters [J].
Xiao Xi ;
Chen Ying-xu ;
Liang Xin-qiang ;
Lou Li-ping ;
Tang Xian-jin .
CHEMOSPHERE, 2010, 81 (09) :1118-1123
[49]  
Ye J X, 1999, ACTA PHYTOECOLOGICA, V23, P379
[50]  
Yu Z, 1992, ACTA HYDROBIOL, V16, P1