Crystal structure of streptokinase β-domain

被引：30
作者：
Wang, XQ
Tang, J
Hunter, B
Zhang, XJC
机构：
[1] Oklahoma Med Res Fdn, Crystallog Program, Oklahoma City, OK 73104 USA
[2] Oklahoma Med Res Fdn, Prot Studies Program, Oklahoma City, OK 73104 USA
[3] Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73104 USA
来源：
FEBS LETTERS | 1999年 / 459卷 / 01期
关键词：
streptokinase; plasminogen; beta-grasp folding; crystal structure;
D O I：
10.1016/S0014-5793(99)01214-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Streptokinase, a 47 kDa secreted protein of hemolytic strains of streptococci, is a human plasminogen activator and contains three structural domains linked by flexible loops. We describe here the crystal structure of the isolated streptokinase middle (SK beta) domain determined at 2.4 Angstrom resolution, Among the functionally important structural features is a putative binding site for a kringle domain of plasminogen located at the tip of a fully exposed hairpin loop. The distribution of genetically conserved residues of SK beta is strongly correlated with their functions. The extensive interface of the SK beta dimer suggests that such dimers may also exist in solution for free SK beta. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：85 / 89
页数：5
相关论文
共 31 条
  • [21] Mapping of the plasminogen binding site of streptokinase with short synthetic peptides
    Nihalani, D
    Raghava, GPS
    Sahni, G
    [J]. PROTEIN SCIENCE, 1997, 6 (06) : 1284 - 1292
  • [22] Processing of X-ray diffraction data collected in oscillation mode
    Otwinowski, Z
    Minor, W
    [J]. MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 : 307 - 326
  • [23] The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action
    Parry, MAA
    Fernandez-Catalan, C
    Bergner, A
    Huber, R
    Hopfner, KP
    Schlott, B
    Gührs, KH
    Bode, W
    [J]. NATURE STRUCTURAL BIOLOGY, 1998, 5 (10) : 917 - 923
  • [24] SIMILARITY OF 3-DIMENSIONAL STRUCTURE BETWEEN IMMUNOGLOBULIN DOMAIN AND COPPER, ZINC SUPEROXIDE-DISMUTASE SUBUNIT
    RICHARDSON, JS
    RICHARDSON, DC
    THOMAS, KA
    SILVERTON, EW
    DAVIES, DR
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1976, 102 (02) : 221 - 235
  • [25] RODRIGUEZ P, 1995, EUR J BIOCHEM, V229, P83, DOI 10.1111/j.1432-1033.1995.0083l.x
  • [26] THE STREPTOKINASE DOMAIN RESPONSIBLE FOR PLASMINOGEN BINDING
    RODRIGUEZ, P
    FUENTES, D
    MUNOZ, E
    RIVERO, D
    ORTA, D
    ALBURQUERQUE, S
    PEREZ, S
    BESADA, V
    HERRERA, L
    [J]. FIBRINOLYSIS, 1994, 8 (05) : 276 - 285
  • [27] BINDING OF STREPTOKINASE AND STAPHYLOKINASE TO PLASMINOGEN
    RODRIGUEZ, P
    COLLEN, D
    LIJNEN, HR
    [J]. FIBRINOLYSIS, 1995, 9 (05) : 298 - 303
  • [28] SUMMARIA L, 1976, J BIOL CHEM, V251, P5810
  • [29] Mapping of the antigenic regions of streptokinase in humans after streptokinase therapy
    Torréns, I
    Reyes, O
    Ojalvo, AG
    Seralena, A
    Chinea, G
    Cruz, LJ
    de la Fuente, J
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 259 (01) : 162 - 168
  • [30] Crystal structure of the catalytic domain of human plasmin complexed with streptokinase
    Wang, XQ
    Lin, XL
    Loy, JA
    Tang, J
    Zhang, XJC
    [J]. SCIENCE, 1998, 281 (5383) : 1662 - 1665
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