Solid phase synthesis, cloning and expression of a synthetic gene encoding a collagen-like peptide

被引：0

作者：

Bruninghoff, A ^{[1
]}

Schlosser, S ^{[1
]}

Groger, G ^{[1
]}

Ortigao, JFR ^{[1
]}

Seliger, H ^{[1
]}

机构：

[1] UNIV ULM,SEKT POLYMERE,D-89069 ULM,GERMANY

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 5-6期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A synthetic gene encoding a collagen-like protein (ACMP, artificial cell matrix protein) was designed, constructed and assembled by combined solid phase ligation and PCR-methods. The ACMP-gene (232 bp) was cloned and expressed in an appropiate E. coli expression system. The expressed ACMP (14 kD) was purified by affinity chromatography.

引用

页码：875 / 882

页数：8

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