Functional diversification of the dehydrin gene family in apple and its contribution to cold acclimation during dormancy

被引：19

作者：

Falavigna, Vitor da Silveira ^{[1
]}

Miotto, Yohanna Evelyn ^{[2
]}

Porto, Diogo Denardi ^{[2
]}

Anzanello, Rafael ^{[3
]}

dos Santos, Henrique Pessoa ^{[3
]}

Fialho, Flavio Bello ^{[3
]}

Margis-Pinheiro, Marcia ^{[1
]}

Pasquali, Giancarlo ^{[1
]}

Revers, Luis Fernando ^{[2
]}

机构：

[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Grad Program Cell & Mol Biol, Porto Alegre, RS, Brazil

[2] Empresa Brasileira Pesquisa Agr, Ctr Nacl Pesquisa Uva & Vinho, Lab Plant Mol Genet, Bento Goncalves, Brazil

[3] Empresa Brasileira Pesquisa Agr, Ctr Nacl Pesquisa Uva & Vinho, Lab Plant Physiol, Bento Goncalves, Brazil

来源：

PHYSIOLOGIA PLANTARUM | 2015年 / 155卷 / 03期

关键词：

GENOME-WIDE IDENTIFICATION; BUD DORMANCY; DIFFERENTIAL EXPRESSION; CHILLING NEGATION; PLANT DEHYDRINS; BARK TISSUES; PEACH LEAF; CBF GENE; PROTEINS; HARDINESS;

D O I：

10.1111/ppl.12338

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Dehydrins (DHN) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene expression. However, an in-depth characterization of DHNs during bud dormancy progression is still missing. An extensive in silico characterization of the apple DHN gene family was performed. Additionally, we used five different experiments that generated samples with different dormancy status, including genotypes with contrasting dormancy traits, to analyze how DHN genes are being regulated during bud dormancy progression in apple by real-time quantitative polymerase chain reaction (RT-qPCR). Duplication events took place in the diversification of apple DHN family. Additionally, MdDHN genes presented tissue- and bud dormant-specific expression patterns. Our results indicate that MdDHN genes are highly divergent in function, with overlapping levels, and that their expressions are fine-tuned by the environment during the dormancy process in apple.

引用

页码：315 / 329

页数：15

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[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].